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• W2285745122 abstract "Research Article1 February 1984free access The Schizosaccharomyces pombe sup3-i suppressor recognizes ochre, but not amber codons in vitro and in vivo. H. Hottinger H. Hottinger Search for more papers by this author B. Stadelmann B. Stadelmann Search for more papers by this author D. Pearson D. Pearson Search for more papers by this author D. Frendewey D. Frendewey Search for more papers by this author J. Kohli J. Kohli Search for more papers by this author D. Söll D. Söll Search for more papers by this author H. Hottinger H. Hottinger Search for more papers by this author B. Stadelmann B. Stadelmann Search for more papers by this author D. Pearson D. Pearson Search for more papers by this author D. Frendewey D. Frendewey Search for more papers by this author J. Kohli J. Kohli Search for more papers by this author D. Söll D. Söll Search for more papers by this author Author Information H. Hottinger, B. Stadelmann, D. Pearson, D. Frendewey, J. Kohli and D. Söll The EMBO Journal (1984)3:423-428https://doi.org/10.1002/j.1460-2075.1984.tb01823.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The inefficient suppressor sup3-i of the fission yeast Schizosaccharomyces pombe is an ochre suppressor. Sup3-i was derived from the efficient serine inserting UGA suppressor sup3-e. The cloning and sequencing of the sup3-i gene indicate that the suppressor is different from the parent sup3-e by a C–-T substitution in the sequence coding for the middle position of the anticodon. In vitro translation assays supplemented with purified sup3-i tRNA and programmed with Xenopus globin mRNAs lead to the accumulation of a readthrough product in response to UAA termination signals, but not in response to UGA termination codons. Transformation of Saccharomyces cerevisiae nonsense mutant strains with plasmid DNA carrying the S. pombe sup3-i gene, led to ochre, but not amber or UGA suppression in vivo. Previous ArticleNext Article Volume 3Issue 21 February 1984In this issue RelatedDetailsLoading ..." @default.
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• W2285745122 title "The Schizosaccharomyces pombe sup3-i suppressor recognizes ochre, but not amber codons in vitro and in vivo." @default.
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