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• W2285767109 abstract "Transgenic plants for the production of high-value recombinant complex and/or glycosylated proteins are a promising alternative for conventional systems, such as mammalian cells and bacteria. Many groups use plants as production platform for antibodies and antibody fragments. Here, we describe how bivalent camel-like antibodies can be produced in leaves and seeds. Camel-like antibodies are fusions of the antigen-binding domain of heavy chain camel antibodies (VHH) with an Fc fragment of choice. Transient expression in Nicotiana benthamiana leaves allows the production of VHH-Fc antibodies within a few days after the expression plasmid has been obtained. Generation of stable Arabidopsis thaliana transformants allows production of scalable amounts of VHH-Fc antibodies in seeds within a year. Further, we describe how the in planta-produced VHH-Fc antibodies can be quantified by Western blot analysis with Fc-specific antibodies." @default.
• W2285767109 created "2016-06-24" @default.
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• W2285767109 date "2012-01-01" @default.
• W2285767109 modified "2023-09-26" @default.
• W2285767109 title "Production of Camel-Like Antibodies in Plants" @default.
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• W2285767109 doi "https://doi.org/10.1007/978-1-61779-968-6_19" @default.
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