FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2285982287> ?p ?o ?g. }

• W2285982287 endingPage "8" @default.
• W2285982287 startingPage "8" @default.
• W2285982287 abstract "Purpose: To characterize molecular weight fractions of bovine corneal endothelial cell conditioned medium (CM) supporting retinal pigment epithelium (RPE) cell survival on aged and age-related macular degeneration (AMD) Bruch's membrane. Methods: CM was subject to size separation using centrifugal filters. Retentate and filtrate fractions were tested for bioactivity by analyzing RPE survival on submacular Bruch's membrane of aged and AMD donor eyes and behavior on collagen I-coated tissue culture wells. Protein and peptide composition of active fractions was determined by mass spectrometry. Results: Two bioactive fractions, 3-kDa filtrate and a 10-50–kDa fraction, were necessary for RPE survival on aged and AMD Bruch's membrane. The 3-kDa filtrate, but not the 10-50–kDa fraction, supported RPE growth on collagen 1‐coated tissue culture plates. Mass spectrometry of the 10-50–kDa fraction identified 175 extracellular proteins, including growth factors and extracellular matrix molecules. Transforming growth factor (TGF)β-2 was identified as unique to active CM. Peptides representing 29 unique proteins were identified in the 3-KDa filtrate. Conclusions: These results indicate there is a minimum of two bioactive molecules in CM, one found in the 3-kDa filtrate and one in the 10-50–kDa fraction, and that bioactive molecules in both fractions must be present to ensure RPE survival on Bruch's membrane. Mass spectrometry analysis suggested proteins to test in future studies to identify proteins that may contribute to CM bioactivity. Translational Relevance: Results of this study are the first steps in development of an adjunct to cell-based therapy to ensure cell transplant survival and functionality in AMD patients." @default.
• W2285982287 created "2016-06-24" @default.
• W2285982287 creator A5034086259 @default.
• W2285982287 creator A5038228952 @default.
• W2285982287 creator A5042152123 @default.
• W2285982287 creator A5046775442 @default.
• W2285982287 creator A5053328227 @default.
• W2285982287 creator A5054725771 @default.
• W2285982287 creator A5080023639 @default.
• W2285982287 date "2016-02-22" @default.
• W2285982287 modified "2023-10-18" @default.
• W2285982287 title "Two Bioactive Molecular Weight Fractions of a Conditioned Medium Enhance RPE Cell Survival on Age-Related Macular Degeneration and Aged Bruch's Membrane" @default.
• W2285982287 cites W1598189106 @default.
• W2285982287 cites W1705739557 @default.
• W2285982287 cites W1965909442 @default.
• W2285982287 cites W1967814530 @default.
• W2285982287 cites W1969583812 @default.
• W2285982287 cites W1971010914 @default.
• W2285982287 cites W1973321912 @default.
• W2285982287 cites W1992425768 @default.
• W2285982287 cites W1992915966 @default.
• W2285982287 cites W1993922882 @default.
• W2285982287 cites W1995693160 @default.
• W2285982287 cites W1996353160 @default.
• W2285982287 cites W2007775311 @default.
• W2285982287 cites W2009298572 @default.
• W2285982287 cites W2009661588 @default.
• W2285982287 cites W2009789841 @default.
• W2285982287 cites W2011311545 @default.
• W2285982287 cites W2016602601 @default.
• W2285982287 cites W2020955051 @default.
• W2285982287 cites W2024927304 @default.
• W2285982287 cites W2030068872 @default.
• W2285982287 cites W2034372633 @default.
• W2285982287 cites W2034729257 @default.
• W2285982287 cites W2034998367 @default.
• W2285982287 cites W2035162736 @default.
• W2285982287 cites W2041883897 @default.
• W2285982287 cites W2044951287 @default.
• W2285982287 cites W2049725677 @default.
• W2285982287 cites W2063695842 @default.
• W2285982287 cites W2065716546 @default.
• W2285982287 cites W2066888800 @default.
• W2285982287 cites W2067791322 @default.
• W2285982287 cites W2070403771 @default.
• W2285982287 cites W2072816262 @default.
• W2285982287 cites W2074766242 @default.
• W2285982287 cites W2081137133 @default.
• W2285982287 cites W2082647396 @default.
• W2285982287 cites W2084247690 @default.
• W2285982287 cites W2085603287 @default.
• W2285982287 cites W2087066472 @default.
• W2285982287 cites W2088019834 @default.
• W2285982287 cites W2108347444 @default.
• W2285982287 cites W2108491787 @default.
• W2285982287 cites W2114051327 @default.
• W2285982287 cites W2126073113 @default.
• W2285982287 cites W2128421857 @default.
• W2285982287 cites W2141163297 @default.
• W2285982287 cites W2149884610 @default.
• W2285982287 cites W2151056029 @default.
• W2285982287 cites W2166840324 @default.
• W2285982287 cites W2322363882 @default.
• W2285982287 cites W2397048606 @default.
• W2285982287 cites W48458134 @default.
• W2285982287 cites W52995325 @default.
• W2285982287 cites W80027751 @default.
• W2285982287 doi "https://doi.org/10.1167/tvst.5.1.8" @default.
• W2285982287 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4771074" @default.
• W2285982287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26933521" @default.
• W2285982287 hasPublicationYear "2016" @default.
• W2285982287 type Work @default.
• W2285982287 sameAs 2285982287 @default.
• W2285982287 citedByCount "10" @default.
• W2285982287 countsByYear W22859822872018 @default.
• W2285982287 countsByYear W22859822872019 @default.
• W2285982287 countsByYear W22859822872021 @default.
• W2285982287 countsByYear W22859822872023 @default.
• W2285982287 crossrefType "journal-article" @default.
• W2285982287 hasAuthorship W2285982287A5034086259 @default.
• W2285982287 hasAuthorship W2285982287A5038228952 @default.
• W2285982287 hasAuthorship W2285982287A5042152123 @default.
• W2285982287 hasAuthorship W2285982287A5046775442 @default.
• W2285982287 hasAuthorship W2285982287A5053328227 @default.
• W2285982287 hasAuthorship W2285982287A5054725771 @default.
• W2285982287 hasAuthorship W2285982287A5080023639 @default.
• W2285982287 hasBestOaLocation W22859822871 @default.
• W2285982287 hasConcept C118487528 @default.
• W2285982287 hasConcept C181199279 @default.
• W2285982287 hasConcept C185592680 @default.
• W2285982287 hasConcept C189165786 @default.
• W2285982287 hasConcept C2776403814 @default.
• W2285982287 hasConcept C2776582655 @default.
• W2285982287 hasConcept C2778024200 @default.
• W2285982287 hasConcept C2780827179 @default.
• W2285982287 hasConcept C55493867 @default.
• W2285982287 hasConcept C58121356 @default.
• W2285982287 hasConcept C71924100 @default.

• next