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- W2286173690 abstract "Temperature-sensitive mutants producing L-serine efficiently from glycine were obtained from the facultative methylotroph Pseudomonas MS31. Forty-five mutant strains showed adequate growth on methanol at 30°C but little or no growth at 37°C. Fourteen of these mutants produced L-serine more efficiently than the wild-type strain. The typical mutant strain ts 162 showed a high conversion rate in glycine-to-L-serine when the cultivation temperature was changed from a permissive (30°C) to non-permissive state (38 42°C) together with the addition of glycine and methanol after adequate growth. The mutant strain accumulated 6.8 mg L-serine from 12 mg glycine per ml culture under optimum conditions. The reduction of L-serine degrading activity in the mutant strain seemed to contribute to the high productivity of L-serine." @default.
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- W2286173690 date "1981-01-01" @default.
- W2286173690 modified "2023-10-16" @default.
- W2286173690 title "Production of L-serine from glycine by methanol-utilizing bacteria. part II. L-Serine production by temperature-sensitive mutants of methanol-utilizing bacterium Pseudomonas MS 31." @default.
- W2286173690 doi "https://doi.org/10.1271/bbb1961.45.1425" @default.
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