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- W2286569204 abstract "Probing and understanding the dynamics of biomolecules in crowded media is challenging, requiring the development and application of appropriate tools. We have exploited the advantages of fluorescence correlation spectroscopy (FCS) to examine the movement of various fluorescent biomolecules (GFP, phycoerythrin, BSA, clathrin, dextran, polystyrene beads..) in Ficoll70 solutions prepared at different concentrations. These biomolecules have not only different size and weight but also shape, from globular (GFP, BSA, .) to branched (dextran) to triskelia (clathrin). Changes in the apparent diffusion of these biomolecules with Ficoll concentration appears to be adequately described by a single exponential [~exp(-αc)] with α being dependent on the biomolecule. The changes cannot be attributed to changes of the bulk viscosity of Ficoll, as would be expected from the Stokes-Einstein relation derived for a structureless, continuum viscous fluid. Instead, we use de-Gennes-Langevin-Rondeles model, which takes into account the nanoscopic porous-like structures of the Ficoll solutions. Here, the exponential behavior can be interpreted as Ficoll-biomolecule system being under theta condition." @default.
- W2286569204 created "2016-06-24" @default.
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- W2286569204 date "2009-04-01" @default.
- W2286569204 modified "2023-10-16" @default.
- W2286569204 title "Fluorescence Correlation Spectroscopy study of the diffusion of biomolecules in Ficoll solutions" @default.
- W2286569204 doi "https://doi.org/10.1096/fasebj.23.1_supplement.681.6" @default.
- W2286569204 hasPublicationYear "2009" @default.
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