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- W2286834474 abstract "A dynamic cell immobilization mechanism with standardized procedures for screeningthe surface properties of the sensor cells and the supporting matrix provides a foundation forselection of effective crosslinking agents capable of avoiding formation of large cell aggregates.It will significantly leverage the development of biosensors by enabling quantitative detectionand analysis of sensor signals. Employed in the development of continuous fermentation processes, immobilization of cellsshows promising potentials in providing supports for cells to retain their viability withoutsacrificing physiological characteristics. The specific goal of the research at this stage is toinvestigate the possibilities of supporting the cells with matrices whose surface properties mightoffer specific sites for cell immobilization. Four different fibers, including cotton (a seed fiber),polyester (a synthetic fiber), viscose rayon (a regenerated fiber), and silk (an animal fiber), arebeing studied in conjunction with two types of immobilization techniques: (1) cell adhesion viavan der Waal force and surface charges and (2) covalent bonding with PEI under specificconditions. The charges on the surface of E. coli have been quantified by using zeta potential, ameasure of surface charges by detecting the Doppler shift of a laser beam due to the mobility ofcells between diodes that switch back and forth their positive and negative charges at 60 timesper second. The hydrophobic intensity of cell surfaces will be evaluated by the MicrobialAdhesion To Hydrocarbon (MATH) method. The streaming zeta potential method will beconducted to evaluate the fiber surface charges. Moreover, the effects of covalent bonding onimmobilization efficiency, cell viability, and biosensing characteristics will be evaluated againstthe adhesion mechanisms to provide insights on its applications. The biosensing characteristicsof the cells will be monitored using a luminometer." @default.
- W2286834474 created "2016-06-24" @default.
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- W2286834474 date "2001-01-01" @default.
- W2286834474 modified "2023-09-22" @default.
- W2286834474 title "Integration of a Dynamic Cell Immobilization Mechanism in Biosensor Development" @default.
- W2286834474 doi "https://doi.org/10.13031/2013.7503" @default.
- W2286834474 hasPublicationYear "2001" @default.
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