Matches in SemOpenAlex for { <https://semopenalex.org/work/W2287555992> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2287555992 endingPage "132.18" @default.
- W2287555992 startingPage "132.18" @default.
- W2287555992 abstract "Abstract Recapitulating immunodominant A2-restricted HIV-specific CTLs that do not suppress virus in vivo is unlikely to be an effective vaccine strategy. To detect novel subdominant determinants in HIV-1 Gag, we primed CD8+ T cells from five seronegative donors with autologous dendritic cells transduced to express Gag. T cells were re-stimulated weekly with monocytes pulsed with 123 15-mer overlapping peptides (OLPs) spanning Gag. Responses were identified with OLP matrix pools followed by interrogation at the single OLP level in responsive pools using IFN-γ ELISPOT assays. One OLP (Gag145-159) was recognized by all donors. Of note, this reactivity predominated in three of five T cell cultures. Fine mapping with progressively truncated peptides revealed three overlapping epitopes: RTLNAWVKV (RV9), RTLNAWVKVV (RV10) and TLNAWVKVV (TV9). TV9 is a known epitope that is rarely recognized in vivo. In contrast, RV9 and RV10 are novel. Although the latter bound with lower affinities to HLA-A2 than TV9, RV9- and RV10-cultures were readily generated. RV9- and RV10-T cells were cytotoxic, capable of secreting many cytokines, and suppressive of HIV replication in vitro. In sum, we report two novel naturally processed and presented epitopes in HIV p24 that are recognized by pre-infection T cell repertoires. Further studies are needed to explain why these reactivities are rare during infections and more importantly, whether this conserved region of the HIV proteome has value as a prophylactic vaccine for A2 individuals." @default.
- W2287555992 created "2016-06-24" @default.
- W2287555992 creator A5001109360 @default.
- W2287555992 creator A5010966529 @default.
- W2287555992 creator A5036474729 @default.
- W2287555992 creator A5037285448 @default.
- W2287555992 creator A5062133875 @default.
- W2287555992 creator A5084541302 @default.
- W2287555992 creator A5087670157 @default.
- W2287555992 date "2009-04-01" @default.
- W2287555992 modified "2023-09-26" @default.
- W2287555992 title "Interfering overlapping epitopes contribute to the subdominance of an HLA-A2-Restricted HIV Gag-specific Epitope (132.18)" @default.
- W2287555992 doi "https://doi.org/10.4049/jimmunol.182.supp.132.18" @default.
- W2287555992 hasPublicationYear "2009" @default.
- W2287555992 type Work @default.
- W2287555992 sameAs 2287555992 @default.
- W2287555992 citedByCount "0" @default.
- W2287555992 crossrefType "journal-article" @default.
- W2287555992 hasAuthorship W2287555992A5001109360 @default.
- W2287555992 hasAuthorship W2287555992A5010966529 @default.
- W2287555992 hasAuthorship W2287555992A5036474729 @default.
- W2287555992 hasAuthorship W2287555992A5037285448 @default.
- W2287555992 hasAuthorship W2287555992A5062133875 @default.
- W2287555992 hasAuthorship W2287555992A5084541302 @default.
- W2287555992 hasAuthorship W2287555992A5087670157 @default.
- W2287555992 hasConcept C147483822 @default.
- W2287555992 hasConcept C154317977 @default.
- W2287555992 hasConcept C159047783 @default.
- W2287555992 hasConcept C167672396 @default.
- W2287555992 hasConcept C195616568 @default.
- W2287555992 hasConcept C202751555 @default.
- W2287555992 hasConcept C203014093 @default.
- W2287555992 hasConcept C2522874641 @default.
- W2287555992 hasConcept C2776090121 @default.
- W2287555992 hasConcept C2778190748 @default.
- W2287555992 hasConcept C2778886782 @default.
- W2287555992 hasConcept C2779053233 @default.
- W2287555992 hasConcept C2780195530 @default.
- W2287555992 hasConcept C2781241564 @default.
- W2287555992 hasConcept C3013748606 @default.
- W2287555992 hasConcept C54355233 @default.
- W2287555992 hasConcept C6881719 @default.
- W2287555992 hasConcept C86803240 @default.
- W2287555992 hasConcept C8891405 @default.
- W2287555992 hasConceptScore W2287555992C147483822 @default.
- W2287555992 hasConceptScore W2287555992C154317977 @default.
- W2287555992 hasConceptScore W2287555992C159047783 @default.
- W2287555992 hasConceptScore W2287555992C167672396 @default.
- W2287555992 hasConceptScore W2287555992C195616568 @default.
- W2287555992 hasConceptScore W2287555992C202751555 @default.
- W2287555992 hasConceptScore W2287555992C203014093 @default.
- W2287555992 hasConceptScore W2287555992C2522874641 @default.
- W2287555992 hasConceptScore W2287555992C2776090121 @default.
- W2287555992 hasConceptScore W2287555992C2778190748 @default.
- W2287555992 hasConceptScore W2287555992C2778886782 @default.
- W2287555992 hasConceptScore W2287555992C2779053233 @default.
- W2287555992 hasConceptScore W2287555992C2780195530 @default.
- W2287555992 hasConceptScore W2287555992C2781241564 @default.
- W2287555992 hasConceptScore W2287555992C3013748606 @default.
- W2287555992 hasConceptScore W2287555992C54355233 @default.
- W2287555992 hasConceptScore W2287555992C6881719 @default.
- W2287555992 hasConceptScore W2287555992C86803240 @default.
- W2287555992 hasConceptScore W2287555992C8891405 @default.
- W2287555992 hasIssue "1_Supplement" @default.
- W2287555992 hasLocation W22875559921 @default.
- W2287555992 hasOpenAccess W2287555992 @default.
- W2287555992 hasPrimaryLocation W22875559921 @default.
- W2287555992 hasRelatedWork W1964304738 @default.
- W2287555992 hasRelatedWork W1967647128 @default.
- W2287555992 hasRelatedWork W1986429144 @default.
- W2287555992 hasRelatedWork W2011675001 @default.
- W2287555992 hasRelatedWork W2048968217 @default.
- W2287555992 hasRelatedWork W2052483994 @default.
- W2287555992 hasRelatedWork W2075073205 @default.
- W2287555992 hasRelatedWork W2106871637 @default.
- W2287555992 hasRelatedWork W2287555992 @default.
- W2287555992 hasRelatedWork W3213677581 @default.
- W2287555992 hasVolume "182" @default.
- W2287555992 isParatext "false" @default.
- W2287555992 isRetracted "false" @default.
- W2287555992 magId "2287555992" @default.
- W2287555992 workType "article" @default.