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- W2287646585 abstract "The quantitative analysis of minimal residual disease (MRD) with the use of junctional regions of rearranged immunoglobulin (Ig) and T-cell (TCR) receptor genes as MRD target sequences is a complex, multi-step procedure. It includes: the processing of biological material, the detection, clonality assessment, identification, and selection of optimal MRD target sequences, and MRD analysis with the use of real-time quantitative polymerase chain reaction (RQ-PCR), which includes: probe and primer selection, standard curve preparation, testing the sensitivity of primers, DNA quality and quantity testing, quantitative MRD analysis, and data interpretation. Although this approach is laborious, it requires experienced and qualified personnel, and extensive standardization, it is applicable in more than 95 % of acute lymphoblastic leukemia (ALL) patients and enables MRD assessment with high sensitivity (10−4 – 10−5), which makes it a valuable tool for the management of ALL treatment." @default.
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- W2287646585 date "2012-01-01" @default.
- W2287646585 modified "2023-09-23" @default.
- W2287646585 title "Analysis of Minimal Residual Disease with the Use of Rearrangements of Ig/TCR Genes Through RQ-PCR" @default.
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- W2287646585 doi "https://doi.org/10.1007/978-3-642-29467-9_23" @default.
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