Matches in SemOpenAlex for { <https://semopenalex.org/work/W2287707215> ?p ?o ?g. }
- W2287707215 endingPage "888" @default.
- W2287707215 startingPage "879" @default.
- W2287707215 abstract "The objective of this study was to study the human ovarian cancer cell line CABA I by means of short tandem repeats (STR) profiling and cytogenetic analysis in order to prevent future misidentification or cross-contamination and verify its stability during in vitro cultivation. To this end, cells at passages 18 and 38 were analyzed using cytogenetic techniques in order to verify possible chromosomal aberrations and the karyotypic evolution of this cell line; GTG-banding and FISH were also performed. For STR analysis, DNA was extracted using the automated extractor MagNA pure and analyzed by means of PowerPlex 16 HS. STR profiles were analyzed by GeneMapper 3.2.1 software. Whereas comparative cytogenetic analysis of CABA I cells at passage 18 and 38 has demonstrated considerable genetic instability, we found that STR profiles were essentially unaltered in both analyzed passages, suggesting that the STR profile is reliable and could be used for the regular authentication of CABA I over time. It should be emphasized, however, that of the 16 loci generally used in human STR profiles, only 3 were properly detectable in CABA I. The data highlight that the CABA I cell line demonstrates an anomalous STR profile that does not fully adjust the criteria currently used for the identification of human cells; in spite of this, it remains stable during the in vitro maintainance. Moreover, the genetic instability of the CABA I cell line overlaps with those observed in vivo in tumor cells, making it a suitable candidate to analyze, in vitro, the peculiar genetic evolution of ovarian cancer cells." @default.
- W2287707215 created "2016-06-24" @default.
- W2287707215 creator A5006143315 @default.
- W2287707215 creator A5038268761 @default.
- W2287707215 creator A5045366127 @default.
- W2287707215 creator A5047996304 @default.
- W2287707215 creator A5053647614 @default.
- W2287707215 creator A5064941388 @default.
- W2287707215 creator A5074129190 @default.
- W2287707215 creator A5081691882 @default.
- W2287707215 date "2016-02-25" @default.
- W2287707215 modified "2023-09-26" @default.
- W2287707215 title "The human ovarian cancer cell line CABA I: A peculiar genetic evolution" @default.
- W2287707215 cites W1219301472 @default.
- W2287707215 cites W1419228847 @default.
- W2287707215 cites W1438018833 @default.
- W2287707215 cites W1502767888 @default.
- W2287707215 cites W1553976553 @default.
- W2287707215 cites W1573227461 @default.
- W2287707215 cites W191220935 @default.
- W2287707215 cites W1964513663 @default.
- W2287707215 cites W1964677287 @default.
- W2287707215 cites W1983200974 @default.
- W2287707215 cites W1983881535 @default.
- W2287707215 cites W2010042331 @default.
- W2287707215 cites W2010960852 @default.
- W2287707215 cites W2018218670 @default.
- W2287707215 cites W2031213320 @default.
- W2287707215 cites W2034440733 @default.
- W2287707215 cites W2060677973 @default.
- W2287707215 cites W2062814183 @default.
- W2287707215 cites W2068875547 @default.
- W2287707215 cites W2074219085 @default.
- W2287707215 cites W2075654179 @default.
- W2287707215 cites W2077902171 @default.
- W2287707215 cites W2117923091 @default.
- W2287707215 cites W2120795322 @default.
- W2287707215 cites W2131703449 @default.
- W2287707215 cites W2135405268 @default.
- W2287707215 cites W2136046675 @default.
- W2287707215 cites W2143999923 @default.
- W2287707215 cites W2156384462 @default.
- W2287707215 cites W2163316512 @default.
- W2287707215 cites W2167358358 @default.
- W2287707215 cites W2255046153 @default.
- W2287707215 cites W2255703111 @default.
- W2287707215 cites W2287690952 @default.
- W2287707215 cites W2408365156 @default.
- W2287707215 cites W2431986850 @default.
- W2287707215 cites W2443915079 @default.
- W2287707215 cites W301924703 @default.
- W2287707215 cites W4251426292 @default.
- W2287707215 doi "https://doi.org/10.3892/ijmm.2016.2501" @default.
- W2287707215 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4790663" @default.
- W2287707215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26934856" @default.
- W2287707215 hasPublicationYear "2016" @default.
- W2287707215 type Work @default.
- W2287707215 sameAs 2287707215 @default.
- W2287707215 citedByCount "2" @default.
- W2287707215 countsByYear W22877072152016 @default.
- W2287707215 countsByYear W22877072152023 @default.
- W2287707215 crossrefType "journal-article" @default.
- W2287707215 hasAuthorship W2287707215A5006143315 @default.
- W2287707215 hasAuthorship W2287707215A5038268761 @default.
- W2287707215 hasAuthorship W2287707215A5045366127 @default.
- W2287707215 hasAuthorship W2287707215A5047996304 @default.
- W2287707215 hasAuthorship W2287707215A5053647614 @default.
- W2287707215 hasAuthorship W2287707215A5064941388 @default.
- W2287707215 hasAuthorship W2287707215A5074129190 @default.
- W2287707215 hasAuthorship W2287707215A5081691882 @default.
- W2287707215 hasBestOaLocation W22877072151 @default.
- W2287707215 hasConcept C104317684 @default.
- W2287707215 hasConcept C121608353 @default.
- W2287707215 hasConcept C180754005 @default.
- W2287707215 hasConcept C2780427987 @default.
- W2287707215 hasConcept C54355233 @default.
- W2287707215 hasConcept C552990157 @default.
- W2287707215 hasConcept C61320498 @default.
- W2287707215 hasConcept C70721500 @default.
- W2287707215 hasConcept C79386861 @default.
- W2287707215 hasConcept C86803240 @default.
- W2287707215 hasConceptScore W2287707215C104317684 @default.
- W2287707215 hasConceptScore W2287707215C121608353 @default.
- W2287707215 hasConceptScore W2287707215C180754005 @default.
- W2287707215 hasConceptScore W2287707215C2780427987 @default.
- W2287707215 hasConceptScore W2287707215C54355233 @default.
- W2287707215 hasConceptScore W2287707215C552990157 @default.
- W2287707215 hasConceptScore W2287707215C61320498 @default.
- W2287707215 hasConceptScore W2287707215C70721500 @default.
- W2287707215 hasConceptScore W2287707215C79386861 @default.
- W2287707215 hasConceptScore W2287707215C86803240 @default.
- W2287707215 hasIssue "4" @default.
- W2287707215 hasLocation W22877072151 @default.
- W2287707215 hasLocation W22877072152 @default.
- W2287707215 hasLocation W22877072153 @default.
- W2287707215 hasOpenAccess W2287707215 @default.
- W2287707215 hasPrimaryLocation W22877072151 @default.
- W2287707215 hasRelatedWork W1582650232 @default.