FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2287760997> ?p ?o ?g. }

• W2287760997 abstract "Anaplasma phagocytophilum is a causative agent of granulocytic anaplasmosis in mammals, which has a broad geographical distribution and a high degree of clinical diversity. Currently, numerous PCR assays have been developed and used for the detection of A. phagocytophilum in various specimens. However, their performance varies. The aim of this study was to evaluate the performance of five nested PCR assays by detection of 363 ruminant and tick samples, and to select the most appropriate methods for the sensitive detection of A. phagocytophilum in environmental or clinical samples. Positive PCR results for A. phagocytophilum were obtained in 75 (20.7 %), 42 (11.6 %) and 19 (5.2 %) specimens with primer sets EC (EC9/EC12a and SSAP2f/SSAP2r), EE (EE1/EE2 and EE3/EE4) and ge (ge3a/ge10r, ge9f/ge2), respectively. The amplification of template DNA with the primer set MSP (MAP4AP5/MSP4AP3, msp4f/msp4r) could not be obtained in both ruminants and ticks, and a low specificity of the EL primers [EL(569)F/EL(1193)R, EL(569)F/EL(1142)R] in tick samples was observed. Our results revealed that the nested PCR with primer set EC complementary to the 16S rRNA gene was the most sensitive assay for detection of A. phagocytophilum in ruminant and tick specimens. A. phagocytophilum was detected in 47 (35.1 %) sheep, 12 (10.4 %) cattle, and 17 (14.9 %) ticks. Two A. phagocytophilum genotypes were identified, that varied between sheep and cattle in sample collection sites. This report provides more valuable information for the diagnosis and management of granulocytic anaplasmosis in China." @default.
• W2287760997 created "2016-06-24" @default.
• W2287760997 creator A5000609629 @default.
• W2287760997 creator A5005356538 @default.
• W2287760997 creator A5009516068 @default.
• W2287760997 creator A5010686284 @default.
• W2287760997 creator A5030919384 @default.
• W2287760997 creator A5031291553 @default.
• W2287760997 creator A5031875473 @default.
• W2287760997 creator A5042088294 @default.
• W2287760997 creator A5052044256 @default.
• W2287760997 creator A5068205683 @default.
• W2287760997 creator A5088282123 @default.
• W2287760997 date "2016-02-24" @default.
• W2287760997 modified "2023-10-11" @default.
• W2287760997 title "Evaluation of different nested PCRs for detection of Anaplasma phagocytophilum in ruminants and ticks" @default.
• W2287760997 cites W1513601800 @default.
• W2287760997 cites W1914131521 @default.
• W2287760997 cites W1931883549 @default.
• W2287760997 cites W1956842074 @default.
• W2287760997 cites W1989357121 @default.
• W2287760997 cites W1995301267 @default.
• W2287760997 cites W1999391928 @default.
• W2287760997 cites W2029506948 @default.
• W2287760997 cites W2042970549 @default.
• W2287760997 cites W2066852984 @default.
• W2287760997 cites W2083417553 @default.
• W2287760997 cites W2084604667 @default.
• W2287760997 cites W2086860446 @default.
• W2287760997 cites W2094697967 @default.
• W2287760997 cites W2095480531 @default.
• W2287760997 cites W2095885801 @default.
• W2287760997 cites W2112503223 @default.
• W2287760997 cites W2115174145 @default.
• W2287760997 cites W2115216655 @default.
• W2287760997 cites W2115319985 @default.
• W2287760997 cites W2122155955 @default.
• W2287760997 cites W2125121305 @default.
• W2287760997 cites W2127795942 @default.
• W2287760997 cites W2127851542 @default.
• W2287760997 cites W2131184698 @default.
• W2287760997 cites W2133657116 @default.
• W2287760997 cites W2149116483 @default.
• W2287760997 cites W2157101456 @default.
• W2287760997 cites W2157372575 @default.
• W2287760997 cites W2161999329 @default.
• W2287760997 cites W2166508628 @default.
• W2287760997 cites W2166558766 @default.
• W2287760997 cites W2188157729 @default.
• W2287760997 cites W4234694178 @default.
• W2287760997 cites W4321429509 @default.
• W2287760997 doi "https://doi.org/10.1186/s12917-016-0663-2" @default.
• W2287760997 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4765105" @default.
• W2287760997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26911835" @default.
• W2287760997 hasPublicationYear "2016" @default.
• W2287760997 type Work @default.
• W2287760997 sameAs 2287760997 @default.
• W2287760997 citedByCount "21" @default.
• W2287760997 countsByYear W22877609972016 @default.
• W2287760997 countsByYear W22877609972017 @default.
• W2287760997 countsByYear W22877609972018 @default.
• W2287760997 countsByYear W22877609972019 @default.
• W2287760997 countsByYear W22877609972020 @default.
• W2287760997 countsByYear W22877609972021 @default.
• W2287760997 countsByYear W22877609972022 @default.
• W2287760997 countsByYear W22877609972023 @default.
• W2287760997 crossrefType "journal-article" @default.
• W2287760997 hasAuthorship W2287760997A5000609629 @default.
• W2287760997 hasAuthorship W2287760997A5005356538 @default.
• W2287760997 hasAuthorship W2287760997A5009516068 @default.
• W2287760997 hasAuthorship W2287760997A5010686284 @default.
• W2287760997 hasAuthorship W2287760997A5030919384 @default.
• W2287760997 hasAuthorship W2287760997A5031291553 @default.
• W2287760997 hasAuthorship W2287760997A5031875473 @default.
• W2287760997 hasAuthorship W2287760997A5042088294 @default.
• W2287760997 hasAuthorship W2287760997A5052044256 @default.
• W2287760997 hasAuthorship W2287760997A5068205683 @default.
• W2287760997 hasAuthorship W2287760997A5088282123 @default.
• W2287760997 hasBestOaLocation W22877609971 @default.
• W2287760997 hasConcept C104317684 @default.
• W2287760997 hasConcept C157431215 @default.
• W2287760997 hasConcept C159047783 @default.
• W2287760997 hasConcept C159654299 @default.
• W2287760997 hasConcept C178790620 @default.
• W2287760997 hasConcept C185592680 @default.
• W2287760997 hasConcept C2776005033 @default.
• W2287760997 hasConcept C2776370125 @default.
• W2287760997 hasConcept C2777479583 @default.
• W2287760997 hasConcept C2777563447 @default.
• W2287760997 hasConcept C2779159148 @default.
• W2287760997 hasConcept C2779620486 @default.
• W2287760997 hasConcept C2781329793 @default.
• W2287760997 hasConcept C42062724 @default.
• W2287760997 hasConcept C42972112 @default.
• W2287760997 hasConcept C49105822 @default.
• W2287760997 hasConcept C54355233 @default.
• W2287760997 hasConcept C71924100 @default.
• W2287760997 hasConcept C86803240 @default.
• W2287760997 hasConceptScore W2287760997C104317684 @default.
• W2287760997 hasConceptScore W2287760997C157431215 @default.

• next