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- W2287776538 abstract "An assay system for measuring an active phosphate uptake in rat jejunal disks has been developed and used to study the rat's response to 1,25-dihydroxyvitamin D3. The active component of phosphate uptake is stimulated by added KCl, fructose, sodium, and CaCl2, but not MgCl2. Half-saturation values obtained with this assay are 0.08 mM (1,25-dihydroxyvitamin D3-treated) and 0.11 mM (D-deficient). The maximal velocities are 9.6 nmol . cm-2 . min-1 (D-deficient). Using a 5-min preincubation and a 15-min incubation, a dose-response curve for 1,25-dihydroxyvitamin D3 shows 6 pmol to be the lowest dose to give a significant response and 60 pmol to be the level at which the maximal response is reached. Measurement of phosphate uptake versus time after dosing with 1,25-dihydroxyvitamin D3 (300 pmol) revealed a biphasic response with peaks at 8 and 36 h and a trough at 20 h." @default.
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- W2287776538 date "1982-12-01" @default.
- W2287776538 modified "2023-09-27" @default.
- W2287776538 title "1,25-Dihydroxyvitamin C3-stimulated active uptake of phosphate by rat jejunum" @default.
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- W2287776538 doi "https://doi.org/10.1152/ajpendo.1982.243.6.e470" @default.
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