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- W228821637 abstract "This chapter describes a formaldehyde-induced fluorescence method that allows demonstration of secondary amines such as adrenaline although the fluorescence yield is lower for the corresponding primary amines. On the other hand, if liquid formaline is used, the adrenaline will diffuse and only the islands of noradrenaline containing cells remain. The visualization of enzymes and other substances with immunohistochemical methods is dependent upon (1) the retention of the compound in question at the proper site in the tissue and (2) the preservation of immunoreactivity. These two requirements are in principle difficult to reconcile as fixation is mostly needed to obtain a good retention and as fixation decreases antigenicity. The specificity of the immunoreaction is dependent of several factors. Firstly, the enzyme preparation used for immunization has to be pure. Secondly, it is difficult to exclude the possibility that cross-reacting enzymes and other transferases may react with phenylethanolamine-N-methyltransferase (PNMT) antisera. The catecholamine synthesizing enzymes are protein molecules and possess antigenic characteristics." @default.
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- W228821637 date "1980-01-01" @default.
- W228821637 modified "2023-10-03" @default.
- W228821637 title "HISTOCHEMICAL IDENTIFICATION OF ADRENALINE CONTAINING CELLS WITH SPECIAL REFERENCE TO NEURONS" @default.
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- W228821637 doi "https://doi.org/10.1016/b978-0-08-025927-7.50008-9" @default.
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