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- W228826877 abstract "Specific primers were designed for p46000 antigen gene and glyceraldehyde-3-phosphate dehydrogenase(GAPDH)gene of Trichinella spiralis and the real-time quantitative RT-PCR with SYBR Green dye was performed to analyze p46000 gene expression profiles at different developmental stages of T.spiralis.Paraffin sections from parasites and infected tissues were prepared and immunofluorescence was utilized to localize p46000 gene product.The results showed that p46000 gene was transcripted at all stages of T.spiralis life cycle,but the peak levels were detected at muscle larvae and adults stages.Although native p46000 was detected in stichosome and on cuticle of the worms during the life cycle of T.spiralis,the most abundant expression of p46000 was observed at adult worms,intestinal L1 and post-encapsule L1 larvae,indicating that the transcription and expression of p46000 gene is temporal and spatial.The antigen could play an important role during the parasitism processes especially in early infection of T.spiralis.These findings provided the possibility that it could associate with immune evasion of T.spiralis." @default.
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- W228826877 date "2009-01-01" @default.
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- W228826877 title "Stage specific transcription and expression of p46000 antigen gene in Trichinella spiralis." @default.
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