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• W2288569372 abstract "Heavy and transition metals have been widely known for their toxicities for centuries. Arsenic and Lead are highly valued in terms of toxicity to living systems due to their high affinity for sulfhydryl (-SH) containing residues. The chemical interactions of Arsenic and Lead with thiols (.SH rich molecules) results in numerous ill-health effects. As a part of this PhD thesis, the chemical status of GSH was determined in the presence of inorganic and organic complexes of lead and arsenic employing simple spectrophotometric analysis and 1H NMR methods.The behavior of EllmanÂ�fs reagent towards the metal-thiol chemistry was initially investigatedto begin with the study of the changed status of thiols resulting from metal-thiol coordination.Chapter 2 describes the use of NMR methods to study the species in solutions. 1H NMRallowed us to identify additional components of the reaction mixture, their relative abundance and consequently the involvement of these additional EllmanÂ�fs based species in the spectrophotometric errors associated with the use of EllmanÂ�fs reagent. Using 1H NMR methods we are able to show that EllmanÂ�fs reagent can exchange with thiolates previously coordinated with lead and arsenic. In the context of this 1H NMR study, some limitations were found to be associated with the use of EllmanÂ�fs reagent in our study. EllmanÂ�fs reagent was found able to react with the thiols being previously attached with arsenic and lead, leading to an over estimation of the thiols in the solution systems. 4,4Â�A’-dithiodipyridine (DTDP) was adapted instead of EllmanÂ�fs reagent for the spectrophotometric determination of the mixtures involving metal-thiols complexes in aqueous solution and biological mixtures after establishing limitations associated with the use EllmanÂ�fs reagent in this capacity of the study.Chapter 3 describes the 1H NMR carried out to study the conditions and ratios of the complexed thiolates (complexes of both arsenic and lead with some important low molecular weight thiols (Glutathione, N. acetyl cysteine and D-Penicillamine). 1H NMR study presented in this chapter provides detailed information about the changes in the chemical status of GSH that might be the basis of this chemical change observed spectrophotometrically in the form of depletion. 1H NMR methods confirmed metal-thiol adducts (i.e., As-SG3 and Pb-SG2) to be the mechanism behind the GSH depletion in the presence of these metal compounds. After establishing the limitation associated with the use of EllmanÂ�fs reagent, 4,4Â�A’- dithiodipyridine was used for the spectrophotometric determination of the unbound thiols in the presence of complexed thiolates in aqueous solutions. In the course study presented in chapter 4, we have spectrophotometrically investigated the reactions of arsenic and lead with sulfhydryl containing thiols i.e., Glutathione, N-Acetyl cysteine and D-Penicillamine in aqueous solutions. In this chapter, the effects of arsenic (Sodium arsenite and Methylarsonous acid) and lead (Lead acetate and Lead acetyl acetonate) on low molecular weight thiols (Glutathione, N. acetyl cysteine and D-Penicillamine) have been spectrophotometerically quantitated in aqueous solutions. Employing influential variables (i.e., different metal concentrations, incubation times and pH ranges) to the study in aqueous solutions, prominent and regular decrease in the thiol levels were caused by each of the inorganic and organic compounds of arsenic and lead in spectrophotometric analysis. Chapter 5 describes the spectrophotometric estimation of important and most abundant low molecular weight thiol (Glutathione) in the whole blood and blood components (cytosolic Fraction and plasma) of human volunteer. In order to improve our understanding of the coordination chemistry of arsenic and lead in whole blood, cytosolic fraction and Plasma, a systematic study of the changes in glutathione (GSH) levels in these biological samples of healthy human volunteers, has been carried out. The effects of the inorganic and organic compounds of arsenic and lead on glutathione status in these biological samples have been spectrophotometerically evaluated by 4,4Â�A’-dithiodipyridine. Chapter 6 describes the spectrophotometric estimation of Glutathione in WBCs (Lymphocytes and Monocytes) isolated from human blood, while the study carried out in chapter 7 describes the estimation of Glutathione in liver isolated from rabbits. Chapter 8 describes the exchange of arsenic (AsIII) and lead (PbII) between Proteins (Albumin) and low molecular weight thiols (Glutathione, N. acetyl cysteine and DPenicillamine). In addition to thiol disulfide exchange reactions, the exchange of arsenic and lead between Proteins (Albumin) and low molecular weight thiols (Glutathione, N. acetyl cysteine and D-Penicillamine) has also been investigated by Column chromatographic methods using EllmanÂ�fs reagent.The exchange behaviour of arsenic and lead between proteins and low molecular weight thiols have been analyzed spectrophotometrically by making use of EllmanÂ�fs reagent. Column chromatographic methods have been used to collect complexed proteins.The sulfhydryls present on the pure and complexed proteins have been estimated spectrophotometerically.The study regarding the thiol disulfide exchange reactions resulted that the low molecular weight thiolates (Reduced glutathione and N-acetylcysteine) take off As(III) and Pb(II) species which are attached to proteins.Results from multiple studies presented in this PhD thesis are consistent to conclude and panticipate that lead and arsenic species are dynamic in nature as in the case of using Ellman’s reagent, thiolates could be removed from the coordination sphere of the arsenic and lead in As(SR)3 and Pb(SR)2 respectively and in the case involving albumin, the slow exchange of lead and arsenic species bound to cys-34 is the basis for a mechanism by which toxic species can become widely distributed around the body." @default.
• W2288569372 created "2016-06-24" @default.
• W2288569372 creator A5050512291 @default.
• W2288569372 date "2014-01-01" @default.
• W2288569372 modified "2023-09-27" @default.
• W2288569372 title "TO STUDY THE EFFECT OF SALTS AND COMPLEXES OF LEAD AND ARSENIC METALS ON THE STATUS OF THIOLS IN BLOOD COMPONENTS, PHARMACOLOGICAL AND TOXICOLOGICAL PERSPECTIVES" @default.
• W2288569372 cites W108801673 @default.
• W2288569372 cites W117147008 @default.
• W2288569372 cites W134636856 @default.
• W2288569372 cites W1410537724 @default.
• W2288569372 cites W1488647065 @default.
• W2288569372 cites W1490490217 @default.
• W2288569372 cites W1492076797 @default.
• W2288569372 cites W1494427387 @default.
• W2288569372 cites W1504311383 @default.
• W2288569372 cites W1504792186 @default.
• W2288569372 cites W1507082667 @default.
• W2288569372 cites W1512606558 @default.
• W2288569372 cites W1512869653 @default.
• W2288569372 cites W1516817503 @default.
• W2288569372 cites W1530872731 @default.
• W2288569372 cites W1531899330 @default.
• W2288569372 cites W1535032604 @default.
• W2288569372 cites W1535350863 @default.
• W2288569372 cites W1547929361 @default.
• W2288569372 cites W1557626025 @default.
• W2288569372 cites W1559359786 @default.
• W2288569372 cites W1566944420 @default.
• W2288569372 cites W1567125753 @default.
• W2288569372 cites W1571762451 @default.
• W2288569372 cites W1581414347 @default.
• W2288569372 cites W1581565952 @default.
• W2288569372 cites W1584508580 @default.
• W2288569372 cites W1598330186 @default.
• W2288569372 cites W1598978469 @default.
• W2288569372 cites W1600359323 @default.
• W2288569372 cites W1607926 @default.
• W2288569372 cites W1707178069 @default.
• W2288569372 cites W1777939053 @default.
• W2288569372 cites W1853068488 @default.
• W2288569372 cites W1870140516 @default.
• W2288569372 cites W1891967301 @default.
• W2288569372 cites W1917211171 @default.
• W2288569372 cites W1919901402 @default.
• W2288569372 cites W1927899726 @default.
• W2288569372 cites W1940656683 @default.
• W2288569372 cites W1944984946 @default.
• W2288569372 cites W1950610522 @default.
• W2288569372 cites W1953101143 @default.
• W2288569372 cites W1963607726 @default.
• W2288569372 cites W1964334470 @default.
• W2288569372 cites W1966484107 @default.
• W2288569372 cites W1968621561 @default.
• W2288569372 cites W1972754197 @default.
• W2288569372 cites W1973238993 @default.
• W2288569372 cites W1973875276 @default.
• W2288569372 cites W1975009131 @default.
• W2288569372 cites W1975403006 @default.
• W2288569372 cites W1975835742 @default.
• W2288569372 cites W1976434985 @default.
• W2288569372 cites W1980125299 @default.
• W2288569372 cites W1981245233 @default.
• W2288569372 cites W1981757532 @default.
• W2288569372 cites W1982022971 @default.
• W2288569372 cites W1982433807 @default.
• W2288569372 cites W1983825154 @default.
• W2288569372 cites W1984238291 @default.
• W2288569372 cites W1988354330 @default.
• W2288569372 cites W1989749016 @default.
• W2288569372 cites W1990079644 @default.
• W2288569372 cites W1990201609 @default.
• W2288569372 cites W1992676492 @default.
• W2288569372 cites W1993338946 @default.
• W2288569372 cites W1995673954 @default.
• W2288569372 cites W1995750280 @default.
• W2288569372 cites W1996311437 @default.
• W2288569372 cites W1997237123 @default.
• W2288569372 cites W1997565724 @default.
• W2288569372 cites W1997730701 @default.
• W2288569372 cites W1998372130 @default.
• W2288569372 cites W1999383641 @default.
• W2288569372 cites W1999909021 @default.
• W2288569372 cites W2001856769 @default.
• W2288569372 cites W2004221135 @default.
• W2288569372 cites W2006370393 @default.
• W2288569372 cites W2007788761 @default.
• W2288569372 cites W2007996640 @default.
• W2288569372 cites W2009917982 @default.
• W2288569372 cites W2010311619 @default.
• W2288569372 cites W2012392743 @default.
• W2288569372 cites W2015267490 @default.
• W2288569372 cites W2016235998 @default.
• W2288569372 cites W2018003739 @default.
• W2288569372 cites W2018539752 @default.
• W2288569372 cites W2019001895 @default.
• W2288569372 cites W2019185666 @default.
• W2288569372 cites W2019397758 @default.
• W2288569372 cites W2019438362 @default.
• W2288569372 cites W2020285880 @default.
• W2288569372 cites W2021436332 @default.

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