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• W2288638618 endingPage "150223" @default.
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• W2288638618 abstract "In Saccharomyces cerevisiae, the mitochondrial carrier family protein Pic2 imports copper into the matrix. Deletion of PIC2 causes defects in mitochondrial copper uptake and copper-dependent growth phenotypes owing to decreased cytochrome c oxidase activity. However, copper import is not completely eliminated in this mutant, so alternative transport systems must exist. Deletion of MRS3, a component of the iron import machinery, also causes a copper-dependent growth defect on non-fermentable carbon. Deletion of both PIC2 and MRS3 led to a more severe respiratory growth defect than either individual mutant. In addition, MRS3 expressed from a high copy number vector was able to suppress the oxygen consumption and copper uptake defects of a strain lacking PIC2. When expressed in Lactococcus lactis, Mrs3 mediated copper and iron import. Finally, a PIC2 and MRS3 double mutant prevented the copper-dependent activation of a heterologously expressed copper sensor in the mitochondrial intermembrane space. Taken together, these data support a role for the iron transporter Mrs3 in copper import into the mitochondrial matrix." @default.
• W2288638618 created "2016-06-24" @default.
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• W2288638618 date "2016-01-01" @default.
• W2288638618 modified "2023-10-14" @default.
• W2288638618 title "Overlap of copper and iron uptake systems in mitochondria in <i>Saccharomyces cerevisiae</i>" @default.
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• W2288638618 doi "https://doi.org/10.1098/rsob.150223" @default.
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