Matches in SemOpenAlex for { <https://semopenalex.org/work/W2289078098> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2289078098 endingPage "76" @default.
- W2289078098 startingPage "166" @default.
- W2289078098 abstract "We compared the effects of methoxamine on alpha 1-adrenergic receptor-mediated phosphorylase activation in rat hepatocytes and rabbit aorta. Although methoxamine is a potent agonist in activating phosphorylase of rabbit aorta, it had little effect in rat hepatocytes. Using the phenoxybenzamine inactivation method, we found that the quantitative relationship between 125I-BE2254 (125I-BE) binding capacity and maximal norepinephrine-stimulated phosphorylase activation was nonlinear in rabbit aorta, whereas it was linear in rat hepatocytes. The potency of methoxamine in inhibiting specific 125I-BE binding is significantly (p less than 0.05) higher in rabbit aorta (Kd, 96.4 +/- 7.7 microM), compared with rat hepatocytes (Kd, 283 +/- 16 microM). However, these quantitative differences could not fully explain the blunted [Ca2+]c and phosphorylase responses to methoxamine in rat hepatocytes. Treatment with chlorethylclonidine dose dependently suppressed 125I-BE binding sites and norepinephrine-induced phosphorylase activation in rat hepatocytes, whereas in rabbit aorta it resulted in only a 31% decrease in 125I-BE binding sites, with little effect on phosphorylase activation. Furthermore, alpha 1-adrenergic receptor-mediated cellular events of phosphatidylinositol (PI) hydrolysis and phosphorylase activation were unaffected by the removal of extracellular Ca2+ in rat hepatocytes, whereas both responses were markedly attenuated in rabbit aorta. The results indicate that two different alpha 1-adrenergic receptor subtypes activate glycogen phosphorylase, through different mechanisms for increasing [Ca2+]c in the two systems. In rat hepatocytes, alpha 1 receptors are closely linked to PI hydrolysis and Ca2+ release from intracellular stores and cause phosphorylase activation. In rabbit aorta, on the other hand, activation of alpha 1 receptors increases [Ca2+]c by Ca2+ influx from the extracellular fluid as well as by Ca2+ release, and both PI hydrolysis and phosphorylase activation are caused mainly by the Ca2+ entry. Methoxamine interacts with both chlorethylclonidine-sensitive and -insensitive alpha 1 receptor subtypes but selectively stimulates the alpha 1 receptor subtype that closely couples with the Ca2+ influx." @default.
- W2289078098 created "2016-06-24" @default.
- W2289078098 creator A5028565031 @default.
- W2289078098 creator A5039560593 @default.
- W2289078098 creator A5064975246 @default.
- W2289078098 creator A5085096016 @default.
- W2289078098 date "1989-07-01" @default.
- W2289078098 modified "2023-10-14" @default.
- W2289078098 title "Glycogen phosphorylase activation by two different alpha 1-adrenergic receptor subtypes: methoxamine selectively stimulates a putative alpha 1-adrenergic receptor subtype (alpha 1a) that couples with Ca2+ influx." @default.
- W2289078098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2546049" @default.
- W2289078098 hasPublicationYear "1989" @default.
- W2289078098 type Work @default.
- W2289078098 sameAs 2289078098 @default.
- W2289078098 citedByCount "34" @default.
- W2289078098 countsByYear W22890780982013 @default.
- W2289078098 crossrefType "journal-article" @default.
- W2289078098 hasAuthorship W2289078098A5028565031 @default.
- W2289078098 hasAuthorship W2289078098A5039560593 @default.
- W2289078098 hasAuthorship W2289078098A5064975246 @default.
- W2289078098 hasAuthorship W2289078098A5085096016 @default.
- W2289078098 hasConcept C126322002 @default.
- W2289078098 hasConcept C134018914 @default.
- W2289078098 hasConcept C159110408 @default.
- W2289078098 hasConcept C170493617 @default.
- W2289078098 hasConcept C185592680 @default.
- W2289078098 hasConcept C204779464 @default.
- W2289078098 hasConcept C2775944032 @default.
- W2289078098 hasConcept C2777499176 @default.
- W2289078098 hasConcept C2778938600 @default.
- W2289078098 hasConcept C2780668150 @default.
- W2289078098 hasConcept C2780745239 @default.
- W2289078098 hasConcept C40465926 @default.
- W2289078098 hasConcept C49453240 @default.
- W2289078098 hasConcept C55493867 @default.
- W2289078098 hasConcept C64943373 @default.
- W2289078098 hasConcept C71924100 @default.
- W2289078098 hasConcept C86803240 @default.
- W2289078098 hasConceptScore W2289078098C126322002 @default.
- W2289078098 hasConceptScore W2289078098C134018914 @default.
- W2289078098 hasConceptScore W2289078098C159110408 @default.
- W2289078098 hasConceptScore W2289078098C170493617 @default.
- W2289078098 hasConceptScore W2289078098C185592680 @default.
- W2289078098 hasConceptScore W2289078098C204779464 @default.
- W2289078098 hasConceptScore W2289078098C2775944032 @default.
- W2289078098 hasConceptScore W2289078098C2777499176 @default.
- W2289078098 hasConceptScore W2289078098C2778938600 @default.
- W2289078098 hasConceptScore W2289078098C2780668150 @default.
- W2289078098 hasConceptScore W2289078098C2780745239 @default.
- W2289078098 hasConceptScore W2289078098C40465926 @default.
- W2289078098 hasConceptScore W2289078098C49453240 @default.
- W2289078098 hasConceptScore W2289078098C55493867 @default.
- W2289078098 hasConceptScore W2289078098C64943373 @default.
- W2289078098 hasConceptScore W2289078098C71924100 @default.
- W2289078098 hasConceptScore W2289078098C86803240 @default.
- W2289078098 hasIssue "1" @default.
- W2289078098 hasLocation W22890780981 @default.
- W2289078098 hasOpenAccess W2289078098 @default.
- W2289078098 hasPrimaryLocation W22890780981 @default.
- W2289078098 hasRelatedWork W1835699834 @default.
- W2289078098 hasRelatedWork W1922812725 @default.
- W2289078098 hasRelatedWork W1980421267 @default.
- W2289078098 hasRelatedWork W2017589268 @default.
- W2289078098 hasRelatedWork W2052453588 @default.
- W2289078098 hasRelatedWork W2053581632 @default.
- W2289078098 hasRelatedWork W2165958118 @default.
- W2289078098 hasRelatedWork W2289078098 @default.
- W2289078098 hasRelatedWork W2315166476 @default.
- W2289078098 hasRelatedWork W2398764952 @default.
- W2289078098 hasVolume "36" @default.
- W2289078098 isParatext "false" @default.
- W2289078098 isRetracted "false" @default.
- W2289078098 magId "2289078098" @default.
- W2289078098 workType "article" @default.