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- W2289086122 abstract "The RapidHIT™ 200 Human Identification System and RapidHIT GlobalFiler® Express kit were evaluated and validated for use with single-source reference samples. It was of primary interest to evaluate the system for its efficacy as an expert system and to estimate a first pass success rate, as well as to identify the technical variables impacting that result. While results indicated that this instrument/kit combination can be used to accurately type single-source buccal samples, substantial variability in sensitivity and intra-color balance were observed, as were multiple artifacts, requiring extensive manual editing of the profiles. Artifacts included dye “blobs” and spectral overlap (pull-up) peaks that often originated from relatively low intensity allele peaks. Reduced intra-color balance, in combination with low sensitivity, occasionally resulted in instances of allelic dropout. Overall, 50% of the buccal samples analyzed in this study would have been successfully typed to give full GlobalFiler® profiles without the need for manual review and editing." @default.
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- W2289086122 date "2016-07-01" @default.
- W2289086122 modified "2023-09-23" @default.
- W2289086122 title "Evaluation of the RapidHIT™ 200 and RapidHIT GlobalFiler ® Express kit for fully automated STR genotyping" @default.
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- W2289086122 doi "https://doi.org/10.1016/j.fsigen.2016.03.001" @default.
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