FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2289224326> ?p ?o ?g. }

• W2289224326 endingPage "1316" @default.
• W2289224326 startingPage "1306" @default.
• W2289224326 abstract "G protein-coupled receptors are of key significance for biomedical research. Streamlined approaches for their efficient recombinant production are of pivotal interest in order to explore their intrinsic conformational dynamics and complex ligand binding behavior. We have systematically optimized the co-translational association and folding of G protein-coupled receptors with defined membranes of nanodiscs by cell-free expression approaches. Each optimization step was quantified and the ligand binding active fraction of the receptor samples could drastically be improved. The strategy was exemplified with a stabilized and a non-stabilized derivative of the turkey beta1-adrenergic receptor. Systematic lipid screens with preformed nanodiscs revealed that generation of ligand binding active conformations of the analyzed beta1-adrenergic receptors strongly depends on lipid charge, flexibility and chain length. The lipid composition of the nanodisc membranes modulates the affinities to a variety of ligands of both receptor derivatives. In addition, the thermostabilization procedure had a significant impact on specific ligand affinities of the receptor and abolished or reduced the binding of certain antagonists. Both receptors were highly stable after purification with optimized nanodisc membranes. The procedure avoids any detergent contact of the receptors and sample production takes less than two days. Moreover, even non-stabilized receptors can be analyzed and their prior purification is not necessary for the formation of nanodisc complexes. The established process appears therefore to be suitable as a new platform for the functional or even structural characterization of recombinant G protein-coupled receptors associated with defined lipid environments." @default.
• W2289224326 created "2016-06-24" @default.
• W2289224326 creator A5025286296 @default.
• W2289224326 creator A5026639692 @default.
• W2289224326 creator A5027282524 @default.
• W2289224326 date "2016-06-01" @default.
• W2289224326 modified "2023-09-27" @default.
• W2289224326 title "Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments" @default.
• W2289224326 cites W1498868646 @default.
• W2289224326 cites W1908561802 @default.
• W2289224326 cites W1955998364 @default.
• W2289224326 cites W1964810993 @default.
• W2289224326 cites W1971793561 @default.
• W2289224326 cites W1980826665 @default.
• W2289224326 cites W1982498753 @default.
• W2289224326 cites W1987959660 @default.
• W2289224326 cites W1990322965 @default.
• W2289224326 cites W1992879374 @default.
• W2289224326 cites W2005574282 @default.
• W2289224326 cites W2021027911 @default.
• W2289224326 cites W2025574943 @default.
• W2289224326 cites W2025764877 @default.
• W2289224326 cites W2030975239 @default.
• W2289224326 cites W203285984 @default.
• W2289224326 cites W2033559770 @default.
• W2289224326 cites W2035176534 @default.
• W2289224326 cites W2050784306 @default.
• W2289224326 cites W2054479516 @default.
• W2289224326 cites W2063759211 @default.
• W2289224326 cites W2066104325 @default.
• W2289224326 cites W2068722080 @default.
• W2289224326 cites W2078534574 @default.
• W2289224326 cites W2082330256 @default.
• W2289224326 cites W2086334871 @default.
• W2289224326 cites W2086795013 @default.
• W2289224326 cites W2089832684 @default.
• W2289224326 cites W2103912693 @default.
• W2289224326 cites W2106335202 @default.
• W2289224326 cites W2109014958 @default.
• W2289224326 cites W2118733371 @default.
• W2289224326 cites W2120389768 @default.
• W2289224326 cites W2120703569 @default.
• W2289224326 cites W2127654342 @default.
• W2289224326 cites W2127739127 @default.
• W2289224326 cites W2139173433 @default.
• W2289224326 cites W2153502397 @default.
• W2289224326 cites W2155350436 @default.
• W2289224326 cites W2159005413 @default.
• W2289224326 cites W2159718916 @default.
• W2289224326 cites W2328928852 @default.
• W2289224326 cites W2334143083 @default.
• W2289224326 cites W2404722019 @default.
• W2289224326 cites W2414428335 @default.
• W2289224326 cites W3025891904 @default.
• W2289224326 cites W4240996555 @default.
• W2289224326 cites W4243870597 @default.
• W2289224326 doi "https://doi.org/10.1016/j.bbamem.2016.02.031" @default.
• W2289224326 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26922884" @default.
• W2289224326 hasPublicationYear "2016" @default.
• W2289224326 type Work @default.
• W2289224326 sameAs 2289224326 @default.
• W2289224326 citedByCount "50" @default.
• W2289224326 countsByYear W22892243262016 @default.
• W2289224326 countsByYear W22892243262017 @default.
• W2289224326 countsByYear W22892243262018 @default.
• W2289224326 countsByYear W22892243262019 @default.
• W2289224326 countsByYear W22892243262020 @default.
• W2289224326 countsByYear W22892243262021 @default.
• W2289224326 countsByYear W22892243262022 @default.
• W2289224326 countsByYear W22892243262023 @default.
• W2289224326 crossrefType "journal-article" @default.
• W2289224326 hasAuthorship W2289224326A5025286296 @default.
• W2289224326 hasAuthorship W2289224326A5026639692 @default.
• W2289224326 hasAuthorship W2289224326A5027282524 @default.
• W2289224326 hasBestOaLocation W22892243261 @default.
• W2289224326 hasConcept C116569031 @default.
• W2289224326 hasConcept C12554922 @default.
• W2289224326 hasConcept C135285700 @default.
• W2289224326 hasConcept C170493617 @default.
• W2289224326 hasConcept C185592680 @default.
• W2289224326 hasConcept C2780880673 @default.
• W2289224326 hasConcept C39944091 @default.
• W2289224326 hasConcept C41625074 @default.
• W2289224326 hasConcept C55493867 @default.
• W2289224326 hasConcept C86803240 @default.
• W2289224326 hasConceptScore W2289224326C116569031 @default.
• W2289224326 hasConceptScore W2289224326C12554922 @default.
• W2289224326 hasConceptScore W2289224326C135285700 @default.
• W2289224326 hasConceptScore W2289224326C170493617 @default.
• W2289224326 hasConceptScore W2289224326C185592680 @default.
• W2289224326 hasConceptScore W2289224326C2780880673 @default.
• W2289224326 hasConceptScore W2289224326C39944091 @default.
• W2289224326 hasConceptScore W2289224326C41625074 @default.
• W2289224326 hasConceptScore W2289224326C55493867 @default.
• W2289224326 hasConceptScore W2289224326C86803240 @default.
• W2289224326 hasFunder F4320320879 @default.
• W2289224326 hasIssue "6" @default.
• W2289224326 hasLocation W22892243261 @default.

• next