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• W2289334920 abstract "To identify functional partners of RAX1 in a large scale, a yeast two-hybrid screen was performed. Two candidates, HAT1 (HD-Zip protein 1) and YAB1 (YABBY1), were shown to interact with RAX1 in yeast and the interactions were confirmed by pull-down assays. Transcripts of HAT1, a HD-ZIP class II transcription factor, accumulated in a domain at the adaxial base of young leaf primordia, very similar to that of RAX1. Phenotypic analysis of hat1-1 knockout mutant as well as hat1-1 rax1-3 double mutants did not reveal any role of HAT1 in the process of axillary meristem formation. This might be due to a functional redundancy of HAT1 with its homologs. Co-localization of YAB1 and RAX1 transcripts in the incipient leaf primordia was demonstrated using consecutive sections for RNA in-situ hybridization. Loss of YAB1 function (fil-8) resulted in a strong reduction in axillary bud formation when plants were grown in short photoperiods. YAB1 was shown to regulate axillary meristem formation in a day-length dependent manner as the branching defect of fil-8 was vanished in long days. The rax1-3 fil-8 double mutant showed a similar branching defect as fil-8. Another putative interactor of RAX1 is AtLAX, which was characterized in detail in this study. AtLAX is the closest homolog of the rice LAX and maize BA1 genes in Arabidopsis. LAX and BA1 are shown to play crucial roles in the establishment of axillary meristems in rice and maize, respectively. LAX, BA1 and AtLAX encode bHLH-type transcription factors with highly conserved bHLH domains. Phenotype analysis of atlax knockout mutants and transgenic plants carrying the AtLAXSRDX dominant repressor showed that: 1) AtLAX plays a role in the formation of axillary meristems; 2) AtLAX functions as a transcriptional activator; and 3) AtLAX probably has no functionally redundant homolog(s). The specific expression pattern of AtLAX at the adaxial domain of leaf primordia and accessory shoot formation in the AtLAX-overexpressing plants further support the role of AtLAX in axillary meristem formation. The idea of an interaction between AtLAX and RAX1, a R2R3-type MYB protein, is derived from the classical interaction model of bHLH and MYB proteins described for several developmental processes. The protein interaction between AtLAX and RAX1 is demonstrated in pull down assays and is supported by a nearly completely overlap of their domains of transcript accumulation. In addition, AtLAX mRNA accumulation is down-regulated in vegetative, but not in reproductive apices of rax1-3 loss-of-function mutants, suggesting that RAX1 regulates AtLAX in the process of axillary meristem initiation during the vegetative stage of development. Finally, analysis of the genetic interaction between AtLAX, RAX1 and LAS (LATERAL SUPPRESSOR) demonstrates that these three regulators are necessary for the formation of axillary meristems during both vegetative and reproductive stages of shoot development." @default.
• W2289334920 created "2016-06-24" @default.
• W2289334920 creator A5059548120 @default.
• W2289334920 date "2008-01-01" @default.
• W2289334920 modified "2023-09-23" @default.
• W2289334920 title "Identification and characterization of interactors of RAX1 controlling shoot branching in Arabidopsis thaliana" @default.
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