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- W2289363375 abstract "The objective of this study was to standardize the testing protocol for aquatic toxicity tests with the nematode Caenorhabditis elegans. Several variables which may be important in determining the test outcome were investigated in a randomized block design. Concentration-response data were obtained on nematodes of various developmental stages exposed to four metals (Cd, Pb, Cu, and Hg) and a water-soluble organic toxicant, sodium Pentachlorophenate (PCP), under conditions of varied solvent medium (with or without salts and with or without a bacterial food source). The endpoints measured were 24 and 96-h mortality, as well as development of larval stages to adulthood and evidence of reproduction. The results suggest that nematodes of various ages respond similarly to a given toxicant for all endpoints measured, although adults cultured from eggs appeared more sensitive than adults cultured from dauer larvae. The most important environmental variable in determining toxicity was the medium in which the tests were conducted. The presence of potassium and sodium salts in the medium significantly (p<0.05) reduced the toxicity of many test samples. The presence of bacteria had little effect on 24-h tests with salts, but was important in 96-h survival and development. Based on sensitivity and ease of handling, adultsmore » cultured from eggs are recommended in both 24-h and 96-h mortality (LC50 value) tests, as well as 96-h reproduction tests.« less" @default.
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- W2289363375 date "1995-12-31" @default.
- W2289363375 modified "2023-09-26" @default.
- W2289363375 title "Effects of developmental stage, salts, and food presence on aquatic toxicological endpoints using Caenorhabditis elegans" @default.
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