Matches in SemOpenAlex for { <https://semopenalex.org/work/W2289492744> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2289492744 abstract "Background: Normal human urothelial (NHU) cells can be induced to differentiate by inhibition of the EGFR pathway and activation of PPARγ, which is considered a master transcription factor (TF) for urothelium. Activation of PPARγ results in up-regulation of downstream TFs, amongst which ELF3 was identified as the most up-regulated by microarray. Different phenotypes of NHU cells (proliferating or differentiated) assessed by expression of differentiation markers were found to resemble different types of urothelial cancer (UC) cell lines. The hypothesis is that the differentiation status in UC cell lines reflects cancer evolution pathways, which are selected to be either retained or lost, and define regulatory pathways that UC cells use for growth.Aim: To understand the role of TFs in regulating urothelial differentiation and pathways regulating proliferation in different UC cell lines.Results: Knockdown of ELF3 in NHU cells affected the expression of ELF3. Functional study also showed an essential role of ELF3 in maintaining an effective barrier. Evidence suggested that PPARγ was coincidentally knocked down in some cells. Over-expression of ELF3 demonstrated increased ELF3 transcript, but had no effect on phenotype.A UC cell line (5637) with low expression of differentiation markers resembled non-differentiated NHU cells. When maintained in medium without serum, 5637 cells relied on PPARγ and GSK3β pathways for growth. By contrast, UMUC9 cells, which resembled differentiated NHU cells, could not proliferate when serum or other growth factors were removed.NHU cells or UC cells maintained in different media (± serum) demonstrated different expressing patterns of PPARγ transcripts and protein isoforms.Conclusions: ELF3 was important in regulating differentiation in NHU cells and may affect the expression of other TFs, but was not alone sufficient to initiate the differentiation programme. The phenotype and signalling pathways associated with proliferation in 5637 and UMUC9 cells may have implications for different subtypes of Muscle Invasive Bladder Cancer (MIBC)." @default.
- W2289492744 created "2016-06-24" @default.
- W2289492744 creator A5031648443 @default.
- W2289492744 date "2015-08-01" @default.
- W2289492744 modified "2023-09-22" @default.
- W2289492744 title "Investigation of Transcription Factors Regulating Urothelial Differentiation and Signalling Pathways involved in Urothelial Cancer Cells" @default.
- W2289492744 cites W1986867477 @default.
- W2289492744 cites W2065667627 @default.
- W2289492744 cites W2086516652 @default.
- W2289492744 cites W2099444378 @default.
- W2289492744 cites W2160285950 @default.
- W2289492744 hasPublicationYear "2015" @default.
- W2289492744 type Work @default.
- W2289492744 sameAs 2289492744 @default.
- W2289492744 citedByCount "0" @default.
- W2289492744 crossrefType "dissertation" @default.
- W2289492744 hasAuthorship W2289492744A5031648443 @default.
- W2289492744 hasConcept C104317684 @default.
- W2289492744 hasConcept C134018914 @default.
- W2289492744 hasConcept C148738053 @default.
- W2289492744 hasConcept C173396325 @default.
- W2289492744 hasConcept C2777540882 @default.
- W2289492744 hasConcept C2910041100 @default.
- W2289492744 hasConcept C502942594 @default.
- W2289492744 hasConcept C54355233 @default.
- W2289492744 hasConcept C62112901 @default.
- W2289492744 hasConcept C77411442 @default.
- W2289492744 hasConcept C81885089 @default.
- W2289492744 hasConcept C86339819 @default.
- W2289492744 hasConcept C86803240 @default.
- W2289492744 hasConcept C95444343 @default.
- W2289492744 hasConceptScore W2289492744C104317684 @default.
- W2289492744 hasConceptScore W2289492744C134018914 @default.
- W2289492744 hasConceptScore W2289492744C148738053 @default.
- W2289492744 hasConceptScore W2289492744C173396325 @default.
- W2289492744 hasConceptScore W2289492744C2777540882 @default.
- W2289492744 hasConceptScore W2289492744C2910041100 @default.
- W2289492744 hasConceptScore W2289492744C502942594 @default.
- W2289492744 hasConceptScore W2289492744C54355233 @default.
- W2289492744 hasConceptScore W2289492744C62112901 @default.
- W2289492744 hasConceptScore W2289492744C77411442 @default.
- W2289492744 hasConceptScore W2289492744C81885089 @default.
- W2289492744 hasConceptScore W2289492744C86339819 @default.
- W2289492744 hasConceptScore W2289492744C86803240 @default.
- W2289492744 hasConceptScore W2289492744C95444343 @default.
- W2289492744 hasLocation W22894927441 @default.
- W2289492744 hasOpenAccess W2289492744 @default.
- W2289492744 hasPrimaryLocation W22894927441 @default.
- W2289492744 hasRelatedWork W1930916494 @default.
- W2289492744 hasRelatedWork W1981952623 @default.
- W2289492744 hasRelatedWork W1982080917 @default.
- W2289492744 hasRelatedWork W1984601277 @default.
- W2289492744 hasRelatedWork W2010140681 @default.
- W2289492744 hasRelatedWork W2022971877 @default.
- W2289492744 hasRelatedWork W2023271047 @default.
- W2289492744 hasRelatedWork W2058110545 @default.
- W2289492744 hasRelatedWork W2070378411 @default.
- W2289492744 hasRelatedWork W2128027626 @default.
- W2289492744 hasRelatedWork W2132311725 @default.
- W2289492744 hasRelatedWork W2317281165 @default.
- W2289492744 hasRelatedWork W2793007520 @default.
- W2289492744 hasRelatedWork W2888436458 @default.
- W2289492744 hasRelatedWork W2909375316 @default.
- W2289492744 hasRelatedWork W2942553874 @default.
- W2289492744 hasRelatedWork W2949734294 @default.
- W2289492744 hasRelatedWork W3101319289 @default.
- W2289492744 hasRelatedWork W3177123221 @default.
- W2289492744 hasRelatedWork W2510837502 @default.
- W2289492744 isParatext "false" @default.
- W2289492744 isRetracted "false" @default.
- W2289492744 magId "2289492744" @default.
- W2289492744 workType "dissertation" @default.