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- W2289523563 abstract "SSAP (Sequence-Specific Amplification Polymorphism) molecular marker has proven to be highly efficient in estimating genetic diversity and determining phylogenetic relationships in plant.Based on the result of SSAP molecular data derived from 10 primer combinations on 28 Diospyros spp.,the influence of restriction endonuclease on the performance of SSAP amplification was conducted.The results showed that when the SSAP analysis was digested by the combination of Mse I and EcoR I restriction endonuclease,moreover,its selective PCR amplification was performed with a retrotransposon primer in combination with either Mse I + 3 or EcoR I + 3,the performance of SSAP amplification with the combination of retrotransposon primer and Mse I + 3 was superior than that with the combination of retrotransposon primer and EcoR I + 3.These results would provide a guidance for high efficient and specific selection of restriction endonuclease in application of SSAP retrotransposon-based molecular marker in analysis of plant germplasm." @default.
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- W2289523563 date "2010-01-01" @default.
- W2289523563 modified "2023-09-23" @default.
- W2289523563 title "Influence of restriction endonuclease on the performance of retrotransposon-based SSAP amplification in Diospyros spp." @default.
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