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- W2289768145 abstract "Ash derived from energy generation is used as a source of minerals in livestock feeds. The microbial biosensor recApr–Luc2 was built to detect genotoxic hazard in recycled ash. Escherichia coli SOS gene (recA, lexA, dinI and umuC) expression in response to cisplatin-induced DNA damage led to the selection of the recA promoter. The biosensor required functional RecA expression to respond to genotoxic heavy metals (Cr > Cd ≈ Pb), and polluted ash induced a strong recApr–Luc2 response. In human liver and intestinal cells, heavy metals induced acute toxicity (Cr > Cd > Pb) at concentrations sufficient to activate recApr–Luc2. Cytostatic effects, including genotoxicity, were cell- and metal-dependent, apart from Cr. In agreement with the recApr–Luc2 bioassay, Cr had the strongest effect in all cells. In conclusion, recApr–Luc2 could be useful for evaluating the genotoxic risk of pollutants present in ash that might be concentrated in animal products and, thus, entering the human food chain." @default.
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- W2289768145 date "2016-08-01" @default.
- W2289768145 modified "2023-10-18" @default.
- W2289768145 title "Biodetection of potential genotoxic pollutants entering the human food chain through ashes used in livestock diets" @default.
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- W2289768145 doi "https://doi.org/10.1016/j.foodchem.2016.02.160" @default.
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