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- W2290144459 abstract "Trans-lesion synthesis polymerases, like DNA Polymerase-η (Pol-η), are essential for cell survival. Pol-η bypasses ultraviolet-induced DNA damages via a two-metal-ion mechanism that assures DNA strand elongation, with formation of the leaving group pyrophosphate (PPi). Recent structural and kinetics studies have shown that Pol-η function depends on the highly flexible and conserved Arg61 and, intriguingly, on a transient third ion resolved at the catalytic site, as lately observed in other nucleic acid-processing metalloenzymes. How these conserved structural features facilitate DNA replication, however, is still poorly understood. Through extended molecular dynamics and free energy simulations, we unravel a highly cooperative and dynamic mechanism for DNA elongation and repair, which is here described by an equilibrium ensemble of structures that connect the reactants to the products in Pol-η catalysis. We reveal that specific conformations of Arg61 help facilitate the recruitment of the incoming base and favor the proper formation of a pre-reactive complex in Pol-η for efficient DNA editing. Also, we show that a third transient metal ion, which acts concertedly with Arg61, serves as an exit shuttle for the leaving PPi. Finally, we discuss how this effective and cooperative mechanism for DNA repair may be shared by other DNA-repairing polymerases." @default.
- W2290144459 created "2016-06-24" @default.
- W2290144459 creator A5023462906 @default.
- W2290144459 creator A5056380742 @default.
- W2290144459 creator A5062592876 @default.
- W2290144459 creator A5065598178 @default.
- W2290144459 date "2016-03-01" @default.
- W2290144459 modified "2023-10-15" @default.
- W2290144459 title "Cooperative motion of a key positively charged residue and metal ions for DNA replication catalyzed by human DNA Polymerase-η" @default.
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- W2290144459 doi "https://doi.org/10.1093/nar/gkw128" @default.
- W2290144459 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4824119" @default.
- W2290144459 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26935581" @default.
- W2290144459 hasPublicationYear "2016" @default.
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