Matches in SemOpenAlex for { <https://semopenalex.org/work/W2290391889> ?p ?o ?g. }
- W2290391889 endingPage "744" @default.
- W2290391889 startingPage "737" @default.
- W2290391889 abstract "Understanding the intricate steps of protein kinase regulation requires characterization of protein-protein interactions between the catalytic subunit, its regulatory partners and the substrate. Fluorescent probes are useful tools with which to study such interactions and to gain insight into their affinities and specificities. Solvatochromic probes, which display changes in their fluorescence emission in response to changes in the polarity of the medium, are particularly attractive. Here we describe conjugation of a switchable fluorescent dye, TP-2Rho, to peptide and protein derivatives of cyclin-dependent kinase 4 (CDK4) and its application to characterization of the interactions between the catalytic subunit of this kinase, its regulatory partner cyclin D1 and a peptide substrate. We demonstrate the sensitivity of TP-2Rho in relation to of those other dyes used for monitoring peptide-protein and protein-protein interactions. Moreover, we show that TP-Rho-labelled peptides can be introduced into living cells to probe endogenous CDK4/cyclin D." @default.
- W2290391889 created "2016-06-24" @default.
- W2290391889 creator A5003131298 @default.
- W2290391889 creator A5018056053 @default.
- W2290391889 creator A5019985028 @default.
- W2290391889 creator A5020151571 @default.
- W2290391889 creator A5034226859 @default.
- W2290391889 creator A5064679818 @default.
- W2290391889 creator A5086244580 @default.
- W2290391889 date "2016-03-17" @default.
- W2290391889 modified "2023-09-25" @default.
- W2290391889 title "TP-2Rho Is a Sensitive Solvatochromic Red-Shifted Probe for Monitoring the Interactions between CDK4 and Cyclin D" @default.
- W2290391889 cites W1969345506 @default.
- W2290391889 cites W1969939692 @default.
- W2290391889 cites W1977431691 @default.
- W2290391889 cites W1985139403 @default.
- W2290391889 cites W1996318954 @default.
- W2290391889 cites W2007031588 @default.
- W2290391889 cites W2007618242 @default.
- W2290391889 cites W2009803588 @default.
- W2290391889 cites W2019127386 @default.
- W2290391889 cites W2026332281 @default.
- W2290391889 cites W2028581135 @default.
- W2290391889 cites W2034749721 @default.
- W2290391889 cites W2038997387 @default.
- W2290391889 cites W2046919270 @default.
- W2290391889 cites W2052221882 @default.
- W2290391889 cites W2057272791 @default.
- W2290391889 cites W2061562811 @default.
- W2290391889 cites W2067988713 @default.
- W2290391889 cites W2070118809 @default.
- W2290391889 cites W2075152619 @default.
- W2290391889 cites W2078942070 @default.
- W2290391889 cites W2080653800 @default.
- W2290391889 cites W2089655089 @default.
- W2290391889 cites W2089657872 @default.
- W2290391889 cites W2096039018 @default.
- W2290391889 cites W2102047529 @default.
- W2290391889 cites W2106198179 @default.
- W2290391889 cites W2108305553 @default.
- W2290391889 cites W2114733818 @default.
- W2290391889 cites W2125330099 @default.
- W2290391889 cites W2143238584 @default.
- W2290391889 cites W2145657367 @default.
- W2290391889 cites W2155638473 @default.
- W2290391889 cites W2167460696 @default.
- W2290391889 cites W2169834283 @default.
- W2290391889 cites W2169927110 @default.
- W2290391889 cites W2315415908 @default.
- W2290391889 cites W2316829517 @default.
- W2290391889 cites W2321291727 @default.
- W2290391889 cites W577099309 @default.
- W2290391889 doi "https://doi.org/10.1002/cbic.201500641" @default.
- W2290391889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26946188" @default.
- W2290391889 hasPublicationYear "2016" @default.
- W2290391889 type Work @default.
- W2290391889 sameAs 2290391889 @default.
- W2290391889 citedByCount "6" @default.
- W2290391889 countsByYear W22903918892019 @default.
- W2290391889 countsByYear W22903918892020 @default.
- W2290391889 countsByYear W22903918892022 @default.
- W2290391889 crossrefType "journal-article" @default.
- W2290391889 hasAuthorship W2290391889A5003131298 @default.
- W2290391889 hasAuthorship W2290391889A5018056053 @default.
- W2290391889 hasAuthorship W2290391889A5019985028 @default.
- W2290391889 hasAuthorship W2290391889A5020151571 @default.
- W2290391889 hasAuthorship W2290391889A5034226859 @default.
- W2290391889 hasAuthorship W2290391889A5064679818 @default.
- W2290391889 hasAuthorship W2290391889A5086244580 @default.
- W2290391889 hasConcept C104292427 @default.
- W2290391889 hasConcept C104317684 @default.
- W2290391889 hasConcept C121332964 @default.
- W2290391889 hasConcept C12554922 @default.
- W2290391889 hasConcept C1491633281 @default.
- W2290391889 hasConcept C175738201 @default.
- W2290391889 hasConcept C184235292 @default.
- W2290391889 hasConcept C185592680 @default.
- W2290391889 hasConcept C18903297 @default.
- W2290391889 hasConcept C2777289219 @default.
- W2290391889 hasConcept C2777361361 @default.
- W2290391889 hasConcept C2779281246 @default.
- W2290391889 hasConcept C2780471494 @default.
- W2290391889 hasConcept C55493867 @default.
- W2290391889 hasConcept C62520636 @default.
- W2290391889 hasConcept C67218372 @default.
- W2290391889 hasConcept C82495950 @default.
- W2290391889 hasConcept C86803240 @default.
- W2290391889 hasConcept C91881484 @default.
- W2290391889 hasConcept C95444343 @default.
- W2290391889 hasConcept C97029542 @default.
- W2290391889 hasConceptScore W2290391889C104292427 @default.
- W2290391889 hasConceptScore W2290391889C104317684 @default.
- W2290391889 hasConceptScore W2290391889C121332964 @default.
- W2290391889 hasConceptScore W2290391889C12554922 @default.
- W2290391889 hasConceptScore W2290391889C1491633281 @default.
- W2290391889 hasConceptScore W2290391889C175738201 @default.
- W2290391889 hasConceptScore W2290391889C184235292 @default.
- W2290391889 hasConceptScore W2290391889C185592680 @default.