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- W2290407129 abstract "Sulfate (SO(4)(2-)) is an abundant component of intestinal mucins and its content is decreased in certain gastrointestinal diseases, including inflammatory bowel disease. In this study, the hyposulfataemic NaS1 sulfate transporter null (Nas1(-/-)) mice were used to investigate the physiological consequences of disturbed sulfate homeostasis on (1) intestinal sulfomucin content and mRNA expression; (2) intestinal permeability and proliferation; (3) dextran sulfate sodium (DSS)-induced colitis; and (4) intestinal barrier function against the bacterial pathogen, Campylobacter jejuni.Intestinal sulfomucins and sialomucins were detected by high iron diamine staining, permeability was assessed by fluorescein isothiocyanate (FITC)-dextran uptake, and proliferation was assessed by 5-bromodeoxyuridine (BrdU) incorporation. Nas1(-/-) and wild-type (Nas1(+/+)) mice received DSS in drinking water, and intestinal damage was assessed by histological, clinical and haematological measurements. Mice were orally inoculated with C jejuni, and intestinal and systemic infection was assessed. Ileal mRNA expression profiles of Nas1(-/-) and Nas1(+/+) mice were determined by cDNA microarrays and validated by quantitative real-time PCR.Nas1(-/-) mice exhibited reduced intestinal sulfomucin content, enhanced intestinal permeability and DSS-induced colitis, and developed systemic infections when challenged orally with C jejuni. The transcriptional profile of 41 genes was altered in Nas1(-/-) mice, with the most upregulated gene being pancreatic lipase-related protein 2 and the most downregulated gene being carbonic anhydrase 1 (Car1).Sulfate homeostasis is essential for maintaining a normal intestinal metabolic state, and hyposulfataemia leads to reduced intestinal sulfomucin content, enhanced susceptibility to toxin-induced colitis and impaired intestinal barrier to bacterial infection." @default.
- W2290407129 created "2016-06-24" @default.
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- W2290407129 date "2009-02-06" @default.
- W2290407129 modified "2023-10-03" @default.
- W2290407129 title "Reduced mucin sulfonation and impaired intestinal barrier function in the hyposulfataemic NaS1 null mouse" @default.
- W2290407129 cites W146115589 @default.
- W2290407129 cites W1491848423 @default.
- W2290407129 cites W150590329 @default.
- W2290407129 cites W1557065670 @default.
- W2290407129 cites W1559827811 @default.
- W2290407129 cites W1589643407 @default.
- W2290407129 cites W1597959509 @default.
- W2290407129 cites W1855226200 @default.
- W2290407129 cites W1870905795 @default.
- W2290407129 cites W1932544076 @default.
- W2290407129 cites W1954354169 @default.
- W2290407129 cites W1966233840 @default.
- W2290407129 cites W1967217097 @default.
- W2290407129 cites W1973955814 @default.
- W2290407129 cites W1974963144 @default.
- W2290407129 cites W1977080919 @default.
- W2290407129 cites W1985442518 @default.
- W2290407129 cites W1988046340 @default.
- W2290407129 cites W1994353048 @default.
- W2290407129 cites W2000807097 @default.
- W2290407129 cites W2009645460 @default.
- W2290407129 cites W2010296582 @default.
- W2290407129 cites W2013444425 @default.
- W2290407129 cites W2017431991 @default.
- W2290407129 cites W2018574528 @default.
- W2290407129 cites W2018620221 @default.
- W2290407129 cites W2019157197 @default.
- W2290407129 cites W2026407856 @default.
- W2290407129 cites W2028843739 @default.
- W2290407129 cites W2031558979 @default.
- W2290407129 cites W2034086037 @default.
- W2290407129 cites W2037022576 @default.
- W2290407129 cites W2039478605 @default.
- W2290407129 cites W2044817923 @default.
- W2290407129 cites W2045137195 @default.
- W2290407129 cites W2046951616 @default.
- W2290407129 cites W2047595952 @default.
- W2290407129 cites W2057542627 @default.
- W2290407129 cites W2069636292 @default.
- W2290407129 cites W2070767044 @default.
- W2290407129 cites W2071833233 @default.
- W2290407129 cites W2072286964 @default.
- W2290407129 cites W2074443029 @default.
- W2290407129 cites W2080785168 @default.
- W2290407129 cites W2082080310 @default.
- W2290407129 cites W2091996810 @default.
- W2290407129 cites W2101203073 @default.
- W2290407129 cites W2107162587 @default.
- W2290407129 cites W2109744701 @default.
- W2290407129 cites W2113474033 @default.
- W2290407129 cites W2124555370 @default.
- W2290407129 cites W2127690112 @default.
- W2290407129 cites W2128890658 @default.
- W2290407129 cites W2132932170 @default.
- W2290407129 cites W2137236448 @default.
- W2290407129 cites W2137917879 @default.
- W2290407129 cites W2146125268 @default.
- W2290407129 cites W2149220361 @default.
- W2290407129 cites W2149489418 @default.
- W2290407129 cites W2150847637 @default.
- W2290407129 cites W2151297727 @default.
- W2290407129 cites W2153176697 @default.
- W2290407129 cites W2154429226 @default.
- W2290407129 cites W2157584041 @default.
- W2290407129 cites W2158572700 @default.
- W2290407129 cites W2169401839 @default.
- W2290407129 cites W2300482125 @default.
- W2290407129 cites W2397681412 @default.
- W2290407129 cites W369079621 @default.
- W2290407129 doi "https://doi.org/10.1136/gut.2007.147595" @default.
- W2290407129 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19201772" @default.
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