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- W2290831996 abstract "In the present study, four fungal isolates from a mangrove soil were screened for their ability to produce α-amylase using solid state fermentation (SSF). Aspergillus flavus proved to be the best α-amylase producer. Various agronomic wastes such as molasses bran, maize meal, rice bran, sugarcane bagasse and wheat bran were used as solid substrates. The highest titer of enzyme activity was obtained when A. flavus was grown on sugarcane bagasse. Different carbon and nitrogen supplements were used to enhance the enzyme production and the highest yield was obtained with soluble starch and yeast extract (1% w/w) as supplements. The optimum pH, temperature, and incubation period for amylase production by the isolate was found to be 6.0, 30°C and 120 h respectively. Partial purification of the amylase using ammonium sulphate fractionation resulted in 5 fold increase in the enzyme activity. Sodium dodecyl sulphate polyacrylamide gel electrophoresis revealed the apparent molecular weight of the enzyme to be 55 kDa. The partially purified enzyme was optimally active at pH 5 and 55°C when 1% of starch and 80.64 µg/ml of enzyme concentrations were used. Presence of Mn 2+ and Ca 2+ salts enhanced the enzyme activity in contrast to Hg 2+ and EDTA, which completely reduced the enzymatic activity. The results obtained in the present study suggest that the mangrove isolate of A. flavus may act as a potent strain for industrial production of α- amylase using SSF with sugarcane bagasse as the substrate." @default.
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- W2290831996 date "2011-01-01" @default.
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- W2290831996 title "Utilization of sugarcane bagasse for solid-state fermentation and characterization of α-amylase from Aspergillus flavus isolated from Muthupettai Mangrove, Tamil Nadu, India." @default.
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