Matches in SemOpenAlex for { <https://semopenalex.org/work/W2290994419> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2290994419 endingPage "49a" @default.
- W2290994419 startingPage "48a" @default.
- W2290994419 abstract "Molecular interactions at biological surfaces are central to numerous physiological functions, such as cell-cell communication, adhesion, or the immune response to pathogens. Most analyses of these interactions require the use of purified proteins, which are studied in suspension or after immobilization on artificial carrier objects. To characterize instead biomolecular dynamics directly at the surface of small biological particles, we have combined optical tweezers with a multi-channel microfluidics chamber. This instrument allows us to expose a laser-trapped particle to a series of microenvironments that are created without walls by laminar flow. We monitor biochemical reactions between the particle surface and supplied ligands by measuring minuscule changes in the particle's response to a well-defined fluidic drag force. Among others, this response is highly sensitive to the particle size. Once a particle is trapped in a typical experiment, the chamber is moved perpendicularly to the main flow direction in a sinusoidal wave to apply a periodic drag force. Using custom-developed software, the resulting particle motion is tracked with a resolution of ∼5 nm at a rate of up to 3,200 frames per second. A linear-systems analysis allows us to monitor the particle radius in different environments with a resolution of a few nanometers. In first measurements, we used this setup to examine the interaction between protein-A and human IgG. We found that at saturation, bound IgG added an apparent thickness of 12.6±1.4(SD) nm to protein-A-coated beads, in agreement with the expected size of human IgG of ∼12 nm. Moreover, we measured an equilibrium constant of KA = 0.115 nM−1 for the protein-A:IgG interaction, in excellent agreement with literature values." @default.
- W2290994419 created "2016-06-24" @default.
- W2290994419 creator A5031743442 @default.
- W2290994419 creator A5073610814 @default.
- W2290994419 date "2016-02-01" @default.
- W2290994419 modified "2023-09-26" @default.
- W2290994419 title "Nanoscale Measurements of Biochemical Interactions at the Surface of Optically Trapped Particles" @default.
- W2290994419 doi "https://doi.org/10.1016/j.bpj.2015.11.327" @default.
- W2290994419 hasPublicationYear "2016" @default.
- W2290994419 type Work @default.
- W2290994419 sameAs 2290994419 @default.
- W2290994419 citedByCount "0" @default.
- W2290994419 crossrefType "journal-article" @default.
- W2290994419 hasAuthorship W2290994419A5031743442 @default.
- W2290994419 hasAuthorship W2290994419A5073610814 @default.
- W2290994419 hasBestOaLocation W22909944191 @default.
- W2290994419 hasConcept C111368507 @default.
- W2290994419 hasConcept C120665830 @default.
- W2290994419 hasConcept C121332964 @default.
- W2290994419 hasConcept C127313418 @default.
- W2290994419 hasConcept C127413603 @default.
- W2290994419 hasConcept C132651336 @default.
- W2290994419 hasConcept C138268822 @default.
- W2290994419 hasConcept C146978453 @default.
- W2290994419 hasConcept C147789679 @default.
- W2290994419 hasConcept C154945302 @default.
- W2290994419 hasConcept C159467904 @default.
- W2290994419 hasConcept C171250308 @default.
- W2290994419 hasConcept C178635117 @default.
- W2290994419 hasConcept C185592680 @default.
- W2290994419 hasConcept C187530423 @default.
- W2290994419 hasConcept C192562407 @default.
- W2290994419 hasConcept C20198109 @default.
- W2290994419 hasConcept C2778517922 @default.
- W2290994419 hasConcept C38652104 @default.
- W2290994419 hasConcept C41008148 @default.
- W2290994419 hasConcept C57879066 @default.
- W2290994419 hasConcept C72921944 @default.
- W2290994419 hasConcept C76563973 @default.
- W2290994419 hasConcept C77066764 @default.
- W2290994419 hasConcept C8673954 @default.
- W2290994419 hasConceptScore W2290994419C111368507 @default.
- W2290994419 hasConceptScore W2290994419C120665830 @default.
- W2290994419 hasConceptScore W2290994419C121332964 @default.
- W2290994419 hasConceptScore W2290994419C127313418 @default.
- W2290994419 hasConceptScore W2290994419C127413603 @default.
- W2290994419 hasConceptScore W2290994419C132651336 @default.
- W2290994419 hasConceptScore W2290994419C138268822 @default.
- W2290994419 hasConceptScore W2290994419C146978453 @default.
- W2290994419 hasConceptScore W2290994419C147789679 @default.
- W2290994419 hasConceptScore W2290994419C154945302 @default.
- W2290994419 hasConceptScore W2290994419C159467904 @default.
- W2290994419 hasConceptScore W2290994419C171250308 @default.
- W2290994419 hasConceptScore W2290994419C178635117 @default.
- W2290994419 hasConceptScore W2290994419C185592680 @default.
- W2290994419 hasConceptScore W2290994419C187530423 @default.
- W2290994419 hasConceptScore W2290994419C192562407 @default.
- W2290994419 hasConceptScore W2290994419C20198109 @default.
- W2290994419 hasConceptScore W2290994419C2778517922 @default.
- W2290994419 hasConceptScore W2290994419C38652104 @default.
- W2290994419 hasConceptScore W2290994419C41008148 @default.
- W2290994419 hasConceptScore W2290994419C57879066 @default.
- W2290994419 hasConceptScore W2290994419C72921944 @default.
- W2290994419 hasConceptScore W2290994419C76563973 @default.
- W2290994419 hasConceptScore W2290994419C77066764 @default.
- W2290994419 hasConceptScore W2290994419C8673954 @default.
- W2290994419 hasIssue "3" @default.
- W2290994419 hasLocation W22909944191 @default.
- W2290994419 hasOpenAccess W2290994419 @default.
- W2290994419 hasPrimaryLocation W22909944191 @default.
- W2290994419 hasRelatedWork W1600370324 @default.
- W2290994419 hasRelatedWork W1996927702 @default.
- W2290994419 hasRelatedWork W2006114956 @default.
- W2290994419 hasRelatedWork W2114559006 @default.
- W2290994419 hasRelatedWork W2117491984 @default.
- W2290994419 hasRelatedWork W2355252195 @default.
- W2290994419 hasRelatedWork W2566838276 @default.
- W2290994419 hasRelatedWork W2591947764 @default.
- W2290994419 hasRelatedWork W3193250096 @default.
- W2290994419 hasRelatedWork W4283800190 @default.
- W2290994419 hasVolume "110" @default.
- W2290994419 isParatext "false" @default.
- W2290994419 isRetracted "false" @default.
- W2290994419 magId "2290994419" @default.
- W2290994419 workType "article" @default.