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• W2291009670 abstract "Human onchocerciasis (river blindness), caused by the filarial nematode Onchocerca volvulus, has been successfully controlled by a single drug, ivermectin, for over 25 years. Ivermectin prevents the disease symptoms of severe itching and visual impairment by killing the microfilarial stage, but does not eliminate the adult parasites, necessitating repeated annual treatments. Mass drug administration with ivermectin does not always break transmission in forest zones and is contraindicated in individuals heavily co-infected with Loa loa, while reports of reduced drug efficacy in Ghana and Cameroon may signal the development of resistance. An alternative treatment for onchocerciasis involves targeting the essential Wolbachia symbiont with tetracycline or its derivatives, which are adulticidal. However, implementation of antibiotic therapy has not occurred on a wide scale due to the prolonged treatment regimen required (several weeks). In the bovine Onchocerca ochengi system, it has been shown previously that prolonged oxytetracycline therapy increases eosinophil counts in intradermal nodules, which kill the adult worms by degranulating on their surface. Here, in an “immunochemotherapeutic” approach, we sought to enhance the efficacy of a short, sub-lethal antibiotic regimen against O. ochengi by prior immunotherapy targeting onchocystatin, an immunomodulatory protein located in the adult female worm cuticle. A key asparagine residue in onchocystatin was mutated to ablate immunomodulatory activity, which has been demonstrated previously to markedly improve the protective efficacy of this vaccine candidate when used as an immunoprophylactic. The immunochemotherapeutic regimen was compared with sub-lethal oxytetracycline therapy alone; onchocystatin immunotherapy alone; a gold-standard prolonged, intermittent oxytetracycline regimen; and no treatment (negative control) in naturally infected Cameroonian cattle. Readouts were collected over one year and comprised adult worm viability, dermal microfilarial density, anti-onchocystatin IgG in sera, and eosinophil counts in nodules. Only the gold-standard antibiotic regimen achieved significant killing of adult worms, a profound reduction in microfilarial load, and a sustained increase in local tissue eosinophilia. A small but statistically significant elevation in anti-onchocystatin IgG was observed for several weeks after immunisation in the immunotherapy-only group, but the antibody response in the immunochemotherapy group was more variable. At 12 weeks post-treatment, only a transient and non-significant increase in eosinophil counts was apparent in the immunochemotherapy group. We conclude that the addition of onchocystatin immunotherapy to a sub-lethal antibiotic regimen is insufficient to induce adulticidal activity, although with booster immunisations or the targeting of additional filarial immunomodulatory proteins, the efficacy of this strategy could be strengthened." @default.
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• W2291009670 date "2015-08-01" @default.
• W2291009670 modified "2023-09-25" @default.
• W2291009670 title "Immunotherapy with mutated onchocystatin fails to enhance the efficacy of a sub-lethal oxytetracycline regimen against Onchocerca ochengi" @default.
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• W2291009670 cites W1962380303 @default.
• W2291009670 cites W1966752641 @default.
• W2291009670 cites W1971179225 @default.
• W2291009670 cites W1974520027 @default.
• W2291009670 cites W1975620626 @default.
• W2291009670 cites W1981104958 @default.
• W2291009670 cites W1987254114 @default.
• W2291009670 cites W1989271238 @default.
• W2291009670 cites W1993835276 @default.
• W2291009670 cites W2005203829 @default.
• W2291009670 cites W2008498547 @default.
• W2291009670 cites W2009051224 @default.
• W2291009670 cites W2009237591 @default.
• W2291009670 cites W2009961740 @default.
• W2291009670 cites W2018562517 @default.
• W2291009670 cites W2019301599 @default.
• W2291009670 cites W2021784254 @default.
• W2291009670 cites W2024107612 @default.
• W2291009670 cites W2025670025 @default.
• W2291009670 cites W2027515342 @default.
• W2291009670 cites W2033700644 @default.
• W2291009670 cites W2035247861 @default.
• W2291009670 cites W2039472587 @default.
• W2291009670 cites W2043826568 @default.
• W2291009670 cites W2045894259 @default.
• W2291009670 cites W2046431598 @default.
• W2291009670 cites W2055623470 @default.
• W2291009670 cites W2063715010 @default.
• W2291009670 cites W2065715351 @default.
• W2291009670 cites W2068691406 @default.
• W2291009670 cites W2074707485 @default.
• W2291009670 cites W2075083605 @default.
• W2291009670 cites W2077672203 @default.
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• W2291009670 cites W2087458512 @default.
• W2291009670 cites W2091443079 @default.
• W2291009670 cites W2093203016 @default.
• W2291009670 cites W2100819009 @default.
• W2291009670 cites W2106982597 @default.
• W2291009670 cites W2109160513 @default.
• W2291009670 cites W2109819054 @default.
• W2291009670 cites W2111853426 @default.
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• W2291009670 cites W2116140918 @default.
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• W2291009670 cites W2117725271 @default.
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• W2291009670 cites W2170246107 @default.
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• W2291009670 doi "https://doi.org/10.1016/j.vetpar.2015.06.005" @default.
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• W2291009670 hasPublicationYear "2015" @default.
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