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• W2291216964 abstract "The aim of the present study was to establish a model of tumor cell growth and visualize HIF-1α overexpression in a nude mouse xenograft model of colorectal cancer (CRC). In the study, HIF-1α lentiviral vector and helper plasmid were co-transfected into 293T packaging cells using a liposome method, and the virus was collected following transfection and used to infect CRC SW480, SW620, LoVo and HCT116 cells. Puromycin was used for the selection and large‑scale amplification of the stable HIF‑1α expression of green fluorescent protein (GFP)-positive cells. HIF‑1α‑expressing cells were injected intraperitoneally into a nude mouse xenograft model, and resulting tumor nodules was separated and confirmed using an inverted fluorescence microscope. The results demonstrated that HIF-1α was not expressed in CRC cells in normoxic conditions. When treated with CoCl2, the expression of HIF‑1α could be induced in all the cancer cell lines, except SW480. HIF-1α was highly expressed following infection with lentiviral particles. Stable expression of HIF‑1α promoted migration in the SW480 cells. Following intraperitoneal injection of nude mice with SW480‑HIF‑1α, a significant number of tumor nodules formed in the intestinal wall compared with the controls (P<0.05). The successful construction of the dual expression HIF‑1α and GFP visualization xenograft model provides a good foundation for the screening of HIF-1α-related functions and for investigating the therapeutic potential of drugs that target HIF-1α." @default.
• W2291216964 created "2016-06-24" @default.
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• W2291216964 date "2016-03-01" @default.
• W2291216964 modified "2023-10-14" @default.
• W2291216964 title "Establishment of a colorectal cancer nude mouse visualization model of HIF-1α overexpression" @default.
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• W2291216964 doi "https://doi.org/10.3892/ol.2016.4287" @default.
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