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- W2291766734 abstract "The present study is the first report of the isolation and molecular typing of Mycobacterium avium subspecies paratuberculosis (Map) in cattle and sheep in Mexicali-Baja California; region with serological history of paratuberculosis in small ruminants. In this study, 5 blood samples of sheep and 1 from cattle showing signs sugestive of paratuberculosis were analyzed; serology results showed positive reaction to the Map ELISA with a reason s/p greater than 70 % for all samples. Fecal culture was also performed, three isolates were obtained , two from sheep and one from cattle, all were identified as Map according to the PCR IS900 and f57 and typified as type C on Map, by testing PCR IS1311 and analysis of endonucleases of restriction (IS1311 PCR-REA). The data obtained in this study confirmed the presence of Map in this region and contribute to the knowledge of its distribution within the country; in addition, confirms the presence of Map type C in cattle and sheep. More studies involving a larger number of animals, as well as the inclusion of techniques that allow for the subtyping of Map are require to learn about the biodiversity of this Mycobacterium" @default.
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- W2291766734 date "2013-01-01" @default.
- W2291766734 modified "2023-09-24" @default.
- W2291766734 title "Caracterización molecular de Mycobacterium avium subespecie paratuberculosis en bovinos y ovinos de Mexicali, Baja California, México Molecular characterization of Mycobacterium avium subspecies paratuberculosis from sheep and cattle in Mexicali, Baja California, Mexico" @default.
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