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- W2291953886 abstract "Enzymatic browning is a chemical spoilage responsible for many losses in fruit and vegetables during their storage. A laboratory scale pulsed electric field machine with the capacity of 450 mL was designed and built (Phase I). Poliphenol oxidase (PPO) extracted from apple (red delicious) was treated with ten different combinations of electric field strengths and times, ranging from 15.6-33.6 kV/cm and 110-448 ms respectively and analyzed by a surface response experimental design .The pulse frequency was set at 12.5 kHz and pulse width at 0.04 ms applied in a positive monopolar square waveform pulse (Phase II). The best electric treatment was compared to a thermal (70 °C × 1 min.), chemical (5 mg/L ascorbic acid) treatment and a control (Phase III).The PPO activity was reduced up to 68% of residual activity when applying field strengths of 24.6 kV/cm×250 ms. The activity of PPO was increased up to 15% when treated at 18.2 kV/cm×110 ms. In Phase III the chemical treatment had a residual activity of PPO of about 41% followed by the thermal treatment with a residual activity of 48%, and last, the electric treatment where the residual activity was 64%. The designed machine was effective in the inhibition of PPO and when" @default.
- W2291953886 created "2016-06-24" @default.
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- W2291953886 date "2015-01-01" @default.
- W2291953886 modified "2023-09-28" @default.
- W2291953886 title "Efectos de pretratamientos en la actividad de polifenol oxidasa extraída de manzana (Malus domestica)" @default.
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- W2291953886 hasPublicationYear "2015" @default.
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