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- W2292095485 abstract "Purpose: To evaluate adhesion of Acanthamoeba trophozoites to different silicone hydrogel contact lens (SHCL) generations with and without multipurpose contact lens care solution (MPS) treatment. Methods: Acanthamoeba lugdunensis L3a trophozoites were inoculated onto discs trimmed from SHCLs: first generation, Air Optix (Lotrafilcon B) with a plasma surface treatment, second generation, Acuvue Oasys (Senofilcon A), which contains an internal wetting agent (Hydraclear), and third generation, Biofinity (Comfilcon A) with no surface treatment. After 18-hour inoculation, the number of adherent trophozoites on SHCLs was counted as the control under phase contrast microscopy. The effects of the 3 different MPSs, Opti-Free Express, ReNu Fresh, and Biotrue, soaking SHCLs for 6 hours, on Acanthamoeba adhesion were analyzed. Scanning electron microscopic examination was performed for assessment of Acanthamoeba attached on the lens surface. Results: Acanthamoeba trophozoites showed greater adhesion to Air Optix than to Acuvue Oasys and Biofinity (P < 0.05). On Air Optix and Acuvue Oasys, the number of adherent Acanthamoeba was significantly reduced compared with the control after treatment with Opti-Free Express (P < 0.05), but not significantly reduced by treatment with ReNu Fresh and Biotrue (P > 0.05). Acanthamoeba did not adhere to Biofinity regardless of MPSs treatment. Attachment of the acanthopodia of Acanthamoeba on the curved ridge of the Air Optix lens surface was observed. Conclusions: Acanthamoeba showed greater affinity for the first-generation SHCL and seemed to be more attached on SHCLs with more ridges. MPS with myristamidopropyl dimethylamine reduced the adhesion rate." @default.
- W2292095485 created "2016-06-24" @default.
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- W2292095485 date "2016-05-01" @default.
- W2292095485 modified "2023-10-18" @default.
- W2292095485 title "Adhesion of Acanthamoeba on Silicone Hydrogel Contact Lenses" @default.
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- W2292095485 doi "https://doi.org/10.1097/ico.0000000000000788" @default.
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