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- W2292240177 abstract "Major histocompatibility complex (MHC) genes are known to be involved in host resistance to a variety of‘ antigens and pathogens. In this report, birds from three genetic groups viz. Bantam, Bantamised White leghorn (BWLH) and White Leghorn (WLH) were typed for the MHC class II (BLβ II family) alleles using polymerase chain reaction- sequence specific primer (PCR-SSP) and their association with immune response was evaluated. Genomic DNA was isolated from blood of 36 birds each of Bantam, BWLH and WLH. A 235 bp, exon-2 region of chicken MHC B-Lβ family was amplified using a set of degenerate primers. The products of the 1st round of amplification were subjected to PCR-SSP to amplify B2, B13, B15, and B21. The target sequences with the exception of B13 allele were amplified, producing 222 bp, 222 bp, 213 bp and 213 B19bp fragments. The study revealed nine genotypes viz., B2B2, B2, B2, B15, B15, B15 B19, B19and B21. Allelic B15B19B15B21B19B19B21B21 and genotypic frequencies differed significantly among the three genetic groups." @default.
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- W2292240177 date "2005-01-01" @default.
- W2292240177 modified "2023-09-24" @default.
- W2292240177 title "Molecular characterization of B-L II family (class II MHC) alleles in three strains of poultry and its association with immune response" @default.
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