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- W2292544939 abstract "Translation of mRNAs by the ribosome is stereospecific, with only l-amino acids being incorporated into the nascent polypeptide chain. This stereospecificity results from the exclusion of d-amino acids at three steps during protein synthesis: (1) the aminoacylation of tRNA by aminoacyl-tRNA synthetases, (2) binding of aminoacyl-tRNAs to EF-Tu, and (3) recognition of aminoacyl-tRNAs by the ribosome. As a first step toward incorporating d-amino acids during protein synthesis, we have altered the enantioselectivity of tyrosyl-tRNA synthetase. This enzyme is unusual among aminoacyl-tRNA synthetases, as it can aminoacylate tRNA with d-tyrosine (albeit at a reduced rate compared to l-tyrosine). To change the enantioselectivity of tyrosyl-tRNA synthetase, we introduced the post-transfer editing domain from Pyrococcus horikoshii phenylalanyl-tRNA synthetase into the connective polypeptide 1 (CP1) domain of Geobacillus stearothermophilus tyrosyl-tRNA synthetase (henceforth designated TyrRS-FRSed). We show that the phenylalanyl-tRNA synthetase editing domain is stereospecific, hydrolyzing l-Tyr-tRNA(Tyr), but not d-Tyr-tRNA(Tyr). We further show that inserting the phenylalanyl-tRNA synthetase editing domain into the CP1 domain of tyrosyl-tRNA synthetase decreases the activity of the synthetic site in tyrosyl-tRNA synthetase. This decrease in activity is critical, as it prevents the rate of synthesis from overwhelming the ability of the editing domain to hydrolyze the l-Tyr-tRNA(Tyr) product. Overall, inserting the phenylalanyl-tRNA synthetase editing domain results in a 2-fold shift in the enantioselectivity of tyrosyl-tRNA synthetase toward the d-Tyr-tRNA(Tyr) product. When a 4-fold excess of d-tyrosine is used, approximately 40% of the tRNA(Tyr) is aminoacylated with d-tyrosine." @default.
- W2292544939 created "2016-06-24" @default.
- W2292544939 creator A5066158244 @default.
- W2292544939 creator A5082513462 @default.
- W2292544939 date "2016-03-02" @default.
- W2292544939 modified "2023-09-24" @default.
- W2292544939 title "Altering the Enantioselectivity of Tyrosyl-tRNA Synthetase by Insertion of a Stereospecific Editing Domain" @default.
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- W2292544939 cites W1894267612 @default.
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- W2292544939 cites W1951639553 @default.
- W2292544939 cites W1965362721 @default.
- W2292544939 cites W1967131638 @default.
- W2292544939 cites W1967448903 @default.
- W2292544939 cites W1969303801 @default.
- W2292544939 cites W1970552944 @default.
- W2292544939 cites W1972192399 @default.
- W2292544939 cites W1973453628 @default.
- W2292544939 cites W1975940326 @default.
- W2292544939 cites W1977033162 @default.
- W2292544939 cites W1978352011 @default.
- W2292544939 cites W1979755475 @default.
- W2292544939 cites W1986495256 @default.
- W2292544939 cites W1988858733 @default.
- W2292544939 cites W1993560994 @default.
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- W2292544939 cites W2002143007 @default.
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- W2292544939 cites W2012058033 @default.
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- W2292544939 cites W2020270026 @default.
- W2292544939 cites W2020356355 @default.
- W2292544939 cites W2021853870 @default.
- W2292544939 cites W2025333274 @default.
- W2292544939 cites W2025666847 @default.
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- W2292544939 cites W2043783701 @default.
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- W2292544939 cites W2051659829 @default.
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- W2292544939 cites W2054507895 @default.
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- W2292544939 cites W208388423 @default.
- W2292544939 cites W2086948774 @default.
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- W2292544939 doi "https://doi.org/10.1021/acs.biochem.5b01167" @default.
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- W2292544939 hasPublicationYear "2016" @default.
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