FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2292554530> ?p ?o ?g. }

• W2292554530 endingPage "497" @default.
• W2292554530 startingPage "491" @default.
• W2292554530 abstract "Ganoderma lucidum (G. lucidum) is an oriental medical mushroom that has been widely used in Asian countries for centuries to prevent and treat different diseases, including cancer. The objective of this study was to investigate the effect of A supercritical-CO2 extract of G. lucidum spores on the transforming growth factor beta 1 (TGF-β1)-induced epithelial–mesenchymal transition (EMT) of cholangiocarcinoma cells. This was an in vitro study with human cholangiocarcinoma TFK-1 cells treated with varying concentrations of G. lucidum. A supercritical-CO2 extract of G. lucidum spores (GLE) was obtained from completely sporoderm-broken germinating G. lucidum spores by supercritical fluid carbon dioxide (SCF-CO2) extraction. GLE pre-incubated with human cholangiocarcinoma TFK-1 cells prior to TGF-β1 treatment (2 ng/ml) for 48 h. Changes in EMT markers were analyzed by western blotting and immunofluorescence. The formation of F-actin stress fibers was assessed via immunostaining with phalloidin and examined using confocal microscopy. Additionally, the effect of the GLE on TGF-β1-induced migration was investigated by a Boyden chamber assay. TGF-β1-induced reduction in E-cadherin expression was associated with a loss of epithelial morphology and cell–cell contact. Concomitant increases in N-cadherin and Fibronectin were evident in predominantly elongated fibroblast-like cells. The GLE suppressed the TGF-β1-induced morphological changes and the changes in cadherin expression, and also inhibited the formation of F-actin stress fibers, which are a hallmark of EMT. The GLE also inhibited TGF-β1-induced migration of TFK-1 cells. Our findings provide new evidence that GLE suppress cholangiocarcinoma migration in vitro through inhibition of TGF-β1-induced EMT. The GLE may be clinically applied in the prevention and/or treatment of cancer metastasis." @default.
• W2292554530 created "2016-06-24" @default.
• W2292554530 creator A5000323703 @default.
• W2292554530 creator A5010078682 @default.
• W2292554530 creator A5021687717 @default.
• W2292554530 creator A5031096137 @default.
• W2292554530 creator A5043467433 @default.
• W2292554530 date "2016-05-01" @default.
• W2292554530 modified "2023-10-18" @default.
• W2292554530 title "A supercritical-CO2 extract of Ganoderma lucidum spores inhibits cholangiocarcinoma cell migration by reversing the epithelial–mesenchymal transition" @default.
• W2292554530 cites W1964081206 @default.
• W2292554530 cites W1971417050 @default.
• W2292554530 cites W1974665704 @default.
• W2292554530 cites W1978615651 @default.
• W2292554530 cites W1979314802 @default.
• W2292554530 cites W1987084974 @default.
• W2292554530 cites W1990411609 @default.
• W2292554530 cites W2007848898 @default.
• W2292554530 cites W2014691022 @default.
• W2292554530 cites W2019878158 @default.
• W2292554530 cites W2020875816 @default.
• W2292554530 cites W2022678344 @default.
• W2292554530 cites W2028145881 @default.
• W2292554530 cites W2029263066 @default.
• W2292554530 cites W2034872549 @default.
• W2292554530 cites W2035515971 @default.
• W2292554530 cites W2054182074 @default.
• W2292554530 cites W2061670770 @default.
• W2292554530 cites W2062374027 @default.
• W2292554530 cites W2069033910 @default.
• W2292554530 cites W2082013762 @default.
• W2292554530 cites W2093938534 @default.
• W2292554530 cites W2094266198 @default.
• W2292554530 cites W2112637007 @default.
• W2292554530 cites W2113221516 @default.
• W2292554530 cites W2121479404 @default.
• W2292554530 cites W2142807621 @default.
• W2292554530 cites W2169964349 @default.
• W2292554530 doi "https://doi.org/10.1016/j.phymed.2016.02.019" @default.
• W2292554530 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27064008" @default.
• W2292554530 hasPublicationYear "2016" @default.
• W2292554530 type Work @default.
• W2292554530 sameAs 2292554530 @default.
• W2292554530 citedByCount "21" @default.
• W2292554530 countsByYear W22925545302017 @default.
• W2292554530 countsByYear W22925545302019 @default.
• W2292554530 countsByYear W22925545302020 @default.
• W2292554530 countsByYear W22925545302021 @default.
• W2292554530 countsByYear W22925545302022 @default.
• W2292554530 countsByYear W22925545302023 @default.
• W2292554530 crossrefType "journal-article" @default.
• W2292554530 hasAuthorship W2292554530A5000323703 @default.
• W2292554530 hasAuthorship W2292554530A5010078682 @default.
• W2292554530 hasAuthorship W2292554530A5021687717 @default.
• W2292554530 hasAuthorship W2292554530A5031096137 @default.
• W2292554530 hasAuthorship W2292554530A5043467433 @default.
• W2292554530 hasBestOaLocation W22925545301 @default.
• W2292554530 hasConcept C104317684 @default.
• W2292554530 hasConcept C137738243 @default.
• W2292554530 hasConcept C1491633281 @default.
• W2292554530 hasConcept C153911025 @default.
• W2292554530 hasConcept C185592680 @default.
• W2292554530 hasConcept C194232998 @default.
• W2292554530 hasConcept C55493867 @default.
• W2292554530 hasConcept C76419328 @default.
• W2292554530 hasConcept C86803240 @default.
• W2292554530 hasConceptScore W2292554530C104317684 @default.
• W2292554530 hasConceptScore W2292554530C137738243 @default.
• W2292554530 hasConceptScore W2292554530C1491633281 @default.
• W2292554530 hasConceptScore W2292554530C153911025 @default.
• W2292554530 hasConceptScore W2292554530C185592680 @default.
• W2292554530 hasConceptScore W2292554530C194232998 @default.
• W2292554530 hasConceptScore W2292554530C55493867 @default.
• W2292554530 hasConceptScore W2292554530C76419328 @default.
• W2292554530 hasConceptScore W2292554530C86803240 @default.
• W2292554530 hasFunder F4320321001 @default.
• W2292554530 hasIssue "5" @default.
• W2292554530 hasLocation W22925545301 @default.
• W2292554530 hasLocation W22925545302 @default.
• W2292554530 hasOpenAccess W2292554530 @default.
• W2292554530 hasPrimaryLocation W22925545301 @default.
• W2292554530 hasRelatedWork W1988605659 @default.
• W2292554530 hasRelatedWork W2029849310 @default.
• W2292554530 hasRelatedWork W2092333222 @default.
• W2292554530 hasRelatedWork W2201991444 @default.
• W2292554530 hasRelatedWork W2301143855 @default.
• W2292554530 hasRelatedWork W2913401826 @default.
• W2292554530 hasRelatedWork W2965482715 @default.
• W2292554530 hasRelatedWork W2972901571 @default.
• W2292554530 hasRelatedWork W3029470805 @default.
• W2292554530 hasRelatedWork W4288859386 @default.
• W2292554530 hasVolume "23" @default.
• W2292554530 isParatext "false" @default.
• W2292554530 isRetracted "false" @default.
• W2292554530 magId "2292554530" @default.
• W2292554530 workType "article" @default.