FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2292818646> ?p ?o ?g. }

• W2292818646 endingPage "e0149751" @default.
• W2292818646 startingPage "e0149751" @default.
• W2292818646 abstract "Background MicroRNAs are small noncoding RNAs about 22 nt long that play key roles in almost all biological processes and diseases. The fluorescent labeling and lipofection are two common methods for changing the levels and locating the position of cellular miRNAs. Despite many studies about the mechanism of DNA/RNA lipofection, little is known about the characteristics, mechanisms and specificity of lipofection of fluorescent-labeled miRNAs. Methods and Results Therefore, miRNAs labeled with different fluorescent dyes were transfected into adherent and suspension cells using lipofection reagent. Then, the non-specific binding and its mechanism were investigated by flow cytometer and laser confocal microscopy. The results showed that miRNAs labeled with Cy5 (cyanine fluorescent dye) could firmly bind to the surface of adherent cells (Hela) and suspended cells (K562) even without lipofection reagent. The binding of miRNAs labeled with FAM (carboxyl fluorescein) to K562 cells was obvious, but it was not significant in Hela cells. After lipofectamine reagent was added, most of the fluorescently labeled miRNAs binding to the surface of Hela cells were transfected into intra-cell because of the high transfection efficiency, however, most of them were still binding to the surface of K562 cells. Moreover, the high-salt buffer which could destroy the electrostatic interactions did not affect the above-mentioned non-specific binding, but the organic solvent which could destroy the hydrophobic interactions eliminated it. Conclusions These results implied that the fluorescent-labeled miRNAs could non-specifically bind to the cell surface by hydrophobic interaction. It would lead to significant errors in the estimation of transfection efficiency only according to the cellular fluorescence intensity. Therefore, other methods to evaluate the transfection efficiency and more appropriate fluorescent dyes should be used according to the cell types for the accuracy of results." @default.
• W2292818646 created "2016-06-24" @default.
• W2292818646 creator A5000119502 @default.
• W2292818646 creator A5004754997 @default.
• W2292818646 creator A5014902238 @default.
• W2292818646 creator A5029072358 @default.
• W2292818646 creator A5056053058 @default.
• W2292818646 creator A5083272185 @default.
• W2292818646 creator A5090270861 @default.
• W2292818646 date "2016-03-01" @default.
• W2292818646 modified "2023-10-14" @default.
• W2292818646 title "The Non-Specific Binding of Fluorescent-Labeled MiRNAs on Cell Surface by Hydrophobic Interaction" @default.
• W2292818646 cites W1801213493 @default.
• W2292818646 cites W1969338185 @default.
• W2292818646 cites W1975332416 @default.
• W2292818646 cites W1980226233 @default.
• W2292818646 cites W1985325702 @default.
• W2292818646 cites W1986137107 @default.
• W2292818646 cites W1996447938 @default.
• W2292818646 cites W2002061154 @default.
• W2292818646 cites W2004739654 @default.
• W2292818646 cites W2012068153 @default.
• W2292818646 cites W2020710960 @default.
• W2292818646 cites W2021414543 @default.
• W2292818646 cites W2023620453 @default.
• W2292818646 cites W2029287872 @default.
• W2292818646 cites W2037483637 @default.
• W2292818646 cites W2047082928 @default.
• W2292818646 cites W2052445951 @default.
• W2292818646 cites W2062595050 @default.
• W2292818646 cites W2070380977 @default.
• W2292818646 cites W2077373908 @default.
• W2292818646 cites W2091220084 @default.
• W2292818646 cites W2091510202 @default.
• W2292818646 cites W2097233439 @default.
• W2292818646 cites W2104544316 @default.
• W2292818646 cites W2115093090 @default.
• W2292818646 cites W2119675020 @default.
• W2292818646 cites W2130400901 @default.
• W2292818646 cites W2133968430 @default.
• W2292818646 cites W2135836598 @default.
• W2292818646 cites W2148273027 @default.
• W2292818646 cites W2157029838 @default.
• W2292818646 cites W2168858738 @default.
• W2292818646 doi "https://doi.org/10.1371/journal.pone.0149751" @default.
• W2292818646 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4773022" @default.
• W2292818646 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26930565" @default.
• W2292818646 hasPublicationYear "2016" @default.
• W2292818646 type Work @default.
• W2292818646 sameAs 2292818646 @default.
• W2292818646 citedByCount "5" @default.
• W2292818646 countsByYear W22928186462016 @default.
• W2292818646 countsByYear W22928186462019 @default.
• W2292818646 countsByYear W22928186462021 @default.
• W2292818646 countsByYear W22928186462022 @default.
• W2292818646 crossrefType "journal-article" @default.
• W2292818646 hasAuthorship W2292818646A5000119502 @default.
• W2292818646 hasAuthorship W2292818646A5004754997 @default.
• W2292818646 hasAuthorship W2292818646A5014902238 @default.
• W2292818646 hasAuthorship W2292818646A5029072358 @default.
• W2292818646 hasAuthorship W2292818646A5056053058 @default.
• W2292818646 hasAuthorship W2292818646A5083272185 @default.
• W2292818646 hasAuthorship W2292818646A5090270861 @default.
• W2292818646 hasBestOaLocation W22928186461 @default.
• W2292818646 hasConcept C104317684 @default.
• W2292818646 hasConcept C121332964 @default.
• W2292818646 hasConcept C12554922 @default.
• W2292818646 hasConcept C145059251 @default.
• W2292818646 hasConcept C1491633281 @default.
• W2292818646 hasConcept C153911025 @default.
• W2292818646 hasConcept C185592680 @default.
• W2292818646 hasConcept C24107716 @default.
• W2292818646 hasConcept C2777324032 @default.
• W2292818646 hasConcept C2777366897 @default.
• W2292818646 hasConcept C2778015030 @default.
• W2292818646 hasConcept C40767141 @default.
• W2292818646 hasConcept C49923114 @default.
• W2292818646 hasConcept C54009773 @default.
• W2292818646 hasConcept C54355233 @default.
• W2292818646 hasConcept C55493867 @default.
• W2292818646 hasConcept C62520636 @default.
• W2292818646 hasConcept C81885089 @default.
• W2292818646 hasConcept C86803240 @default.
• W2292818646 hasConcept C91881484 @default.
• W2292818646 hasConcept C92087593 @default.
• W2292818646 hasConcept C95444343 @default.
• W2292818646 hasConceptScore W2292818646C104317684 @default.
• W2292818646 hasConceptScore W2292818646C121332964 @default.
• W2292818646 hasConceptScore W2292818646C12554922 @default.
• W2292818646 hasConceptScore W2292818646C145059251 @default.
• W2292818646 hasConceptScore W2292818646C1491633281 @default.
• W2292818646 hasConceptScore W2292818646C153911025 @default.
• W2292818646 hasConceptScore W2292818646C185592680 @default.
• W2292818646 hasConceptScore W2292818646C24107716 @default.
• W2292818646 hasConceptScore W2292818646C2777324032 @default.
• W2292818646 hasConceptScore W2292818646C2777366897 @default.
• W2292818646 hasConceptScore W2292818646C2778015030 @default.
• W2292818646 hasConceptScore W2292818646C40767141 @default.

• next