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• W2292918559 abstract "Purpose Current culture schema for human intestinal stem cells (hISCs) frequently rely on a 3D culture system using Matrigel™, a laminin-rich matrix derived from murine sarcoma that is not suitable for clinical use. We have developed a novel 2D culture system for the in vitro expansion of hISCs as an intestinal epithelial monolayer without the use of Matrigel. Methods Cadaveric duodenal samples were processed to isolate intestinal crypts from the mucosa. Crypts were cultured on a thin coat of type I collagen or laminin. Intestinal epithelial monolayers were supported with growth factors to promote self-renewal or differentiation of the hISCs. Proliferating monolayers were sub-cultured every 4–5 days. Results Intestinal epithelial monolayers were capable of long-term cell renewal. Less differentiated monolayers expressed high levels of gene marker LGR5, while more differentiated monolayers had higher expressions of CDX2, MUC2, LYZ, DEF5, and CHGA. Furthermore, monolayers were capable of passaging into a 3D culture system to generate spheroids and enteroids. Conclusion This 2D system is an important step to expand hISCs for further experimental studies and for clinical cell transplantation. Level of Evidence: 1 Experimental" @default.
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• W2292918559 date "2016-06-01" @default.
• W2292918559 modified "2023-09-24" @default.
• W2292918559 title "Long-term renewable human intestinal epithelial stem cells as monolayers: A potential for clinical use" @default.
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• W2292918559 doi "https://doi.org/10.1016/j.jpedsurg.2016.02.074" @default.
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