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• W2293373323 abstract "This study aimed to analyze interactions between oral biofilms and a dental triethylene glycol dimethacrylate (TEGDMA)-based resin infiltration material on enamel. Demineralized enamel specimens (14 days, acidic buffer, pH 5.0) were either infiltrated with a commercial TEGDMA resin and subjected to a three-species biofilm (Streptococcus mutans UAB 159, Streptococcus oralis OMZ 607 and Actinomyces oris OMZ 745) (group 1), applied to the biofilm (group 2), or merely resin infiltrated (group 3). A control group received no treatment (4). Biofilm formation and metabolic activity of biofilms were measured for group (1) and (2) after 24 h CFU and a resazurin assay. Resin biodegradation was measured for group (1) and (3) by high performance liquid chromatography (HPLC) coupled with mass spectrometry after 6 and 24 h incubation. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) images were taken to study the biofilm and material’s autofluorescence in groups (1–4) after 24 h. SEM and CLSM images showed reduced biofilm formation on resin-infiltrated specimens (group 1) compared to group 2, while no biofilm was detectable on groups 3 and 4. CFU data (log10 CFU per mL) of group 1 showed significantly reduced bacterial numbers (p < 0.05) compared to group 2. However, HPLC analysis of TEGDMA leakage after 6 h and 24 h revealed no differences between group 1 and group 3. The results of the current study indicate that freshly resin-infiltrated enamel surfaces show a biofilm reducing effect, while monomer leakage was not affected by bacterial presence. Resin infiltrated enamel surfaces are constantly exposed to the oral microflora. Yet, it is not known how biofilms interact with enamel-penetrated resins and if and to which extent accessory alignments in oral hygiene are needed." @default.
• W2293373323 created "2016-06-24" @default.
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• W2293373323 date "2016-05-01" @default.
• W2293373323 modified "2023-09-26" @default.
• W2293373323 title "Oral biofilm and caries-infiltrant interactions on enamel" @default.
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• W2293373323 doi "https://doi.org/10.1016/j.jdent.2016.03.006" @default.
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