FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2293449318> ?p ?o ?g. }

• W2293449318 abstract "Cardiac myosin binding protein-C (cMyBP-C) phosphorylation is an important regulator of contractile function, however, its contributions to length-dependent (SL) changes in cross-bridge (XB) kinetics is unknown. Therefore, we performed mechanical experiments to quantify contractile function in detergent-skinned ventricular preparations isolated from wild-type (WT) hearts, and hearts expressing non-phosphorylatable cMyBP-C (Ser to Ala substitutions at residues Ser273, Ser282, and Ser302 (i.e., 3SA)), at SL 1.9µm or 2.1µm, prior and following protein kinase A (PKA) treatment. Steady-state force generation measurements revealed a blunting in the length-dependent increase in myofilament Ca2+-sensitivity of force generation (pCa50) following an increase in SL in 3SA skinned myocardium compared to WT skinned myocardium. Dynamic XB behavior was assessed at submaximal Ca2+-activations by imposing an acute rapid stretch of 2% of initial muscle length, and measuring both the magnitudes and rates of resultant phases of force decay due to strain-induced XB detachment and delayed force rise due to recruitment of additional XBs with increased SL (i.e., stretch activation). The magnitude (P2) and rate of XB detachment (krel) following stretch was significantly reduced in 3SA skinned myocardium compared to WT skinned myocardium at short and long SL, and prior to and following PKA treatment. Furthermore, the length-dependent acceleration of krel due to decreased SL that was observed in WT skinned myocardium was abolished in 3SA skinned myocardium. PKA treatment accelerated the rate of XB recruitment (kdf) following stretch at both SL’s in WT but not in 3SA skinned myocardium. The amplitude of the enhancement in force generation above initial pre-stretch steady-state levels (P3) was not different between WT and 3SA skinned myocardium at any condition measured. However, the magnitude of the entire delayed force phase which can dip below initial pre-stretch steady-state levels (Pdf) was significantly lower in 3SA skinned myocardium under all conditions, in part due to a reduced magnitude of XB detachment (P2) in 3SA skinned myocardium compared to WT skinned myocardium. These findings demonstrate that cMyBP-C phospho-ablation regulates SL- and PKA-mediated effects on XB kinetics in the myocardium, which would be expected to contribute to the regulation of the Frank-Starling mechanism." @default.
• W2293449318 created "2016-06-24" @default.
• W2293449318 creator A5022501132 @default.
• W2293449318 creator A5054044055 @default.
• W2293449318 creator A5060914516 @default.
• W2293449318 creator A5086776019 @default.
• W2293449318 date "2016-02-15" @default.
• W2293449318 modified "2023-09-24" @default.
• W2293449318 title "Cardiac Myosin Binding Protein-C Phosphorylation Modulates Myofilament Length-Dependent Activation" @default.
• W2293449318 cites W1156084614 @default.
• W2293449318 cites W1523578038 @default.
• W2293449318 cites W1535326074 @default.
• W2293449318 cites W1615746675 @default.
• W2293449318 cites W1669455160 @default.
• W2293449318 cites W1819240213 @default.
• W2293449318 cites W1887158195 @default.
• W2293449318 cites W1961137574 @default.
• W2293449318 cites W1964747697 @default.
• W2293449318 cites W1973738031 @default.
• W2293449318 cites W1974989982 @default.
• W2293449318 cites W1980685237 @default.
• W2293449318 cites W1983111235 @default.
• W2293449318 cites W1985692345 @default.
• W2293449318 cites W1989456266 @default.
• W2293449318 cites W1996288114 @default.
• W2293449318 cites W2002118598 @default.
• W2293449318 cites W2005562374 @default.
• W2293449318 cites W2008034318 @default.
• W2293449318 cites W2009599957 @default.
• W2293449318 cites W2009762763 @default.
• W2293449318 cites W2011541717 @default.
• W2293449318 cites W2014090392 @default.
• W2293449318 cites W2014534876 @default.
• W2293449318 cites W2019718894 @default.
• W2293449318 cites W2020965064 @default.
• W2293449318 cites W2021919943 @default.
• W2293449318 cites W2022166073 @default.
• W2293449318 cites W2024082573 @default.
• W2293449318 cites W2024852158 @default.
• W2293449318 cites W2025252646 @default.
• W2293449318 cites W2025759932 @default.
• W2293449318 cites W2027759264 @default.
• W2293449318 cites W2031267053 @default.
• W2293449318 cites W2033776976 @default.
• W2293449318 cites W2034423185 @default.
• W2293449318 cites W2037066269 @default.
• W2293449318 cites W2038286775 @default.
• W2293449318 cites W2039328704 @default.
• W2293449318 cites W2042194476 @default.
• W2293449318 cites W2043130547 @default.
• W2293449318 cites W2045141838 @default.
• W2293449318 cites W2046514079 @default.
• W2293449318 cites W2049181319 @default.
• W2293449318 cites W2050313711 @default.
• W2293449318 cites W2050824263 @default.
• W2293449318 cites W2053104183 @default.
• W2293449318 cites W2057865516 @default.
• W2293449318 cites W2058581105 @default.
• W2293449318 cites W2061131459 @default.
• W2293449318 cites W2065758030 @default.
• W2293449318 cites W2070536183 @default.
• W2293449318 cites W2073466565 @default.
• W2293449318 cites W2074438321 @default.
• W2293449318 cites W2075739506 @default.
• W2293449318 cites W2076627399 @default.
• W2293449318 cites W2077486687 @default.
• W2293449318 cites W2078988134 @default.
• W2293449318 cites W2087480421 @default.
• W2293449318 cites W2091650498 @default.
• W2293449318 cites W2091867363 @default.
• W2293449318 cites W2096001874 @default.
• W2293449318 cites W2096569657 @default.
• W2293449318 cites W2100797448 @default.
• W2293449318 cites W2102903858 @default.
• W2293449318 cites W2102987687 @default.
• W2293449318 cites W2105542116 @default.
• W2293449318 cites W2107954466 @default.
• W2293449318 cites W2112194805 @default.
• W2293449318 cites W2115783430 @default.
• W2293449318 cites W2121747051 @default.
• W2293449318 cites W2123486104 @default.
• W2293449318 cites W2126371007 @default.
• W2293449318 cites W2128706302 @default.
• W2293449318 cites W2133187260 @default.
• W2293449318 cites W2137110772 @default.
• W2293449318 cites W2137884062 @default.
• W2293449318 cites W2139949573 @default.
• W2293449318 cites W2140547477 @default.
• W2293449318 cites W2141307759 @default.
• W2293449318 cites W2143515941 @default.
• W2293449318 cites W2150274772 @default.
• W2293449318 cites W2151574637 @default.
• W2293449318 cites W2152768014 @default.
• W2293449318 cites W2153370540 @default.
• W2293449318 cites W2153676036 @default.
• W2293449318 cites W2153888846 @default.
• W2293449318 cites W2157796077 @default.
• W2293449318 cites W2161375436 @default.
• W2293449318 cites W2163385416 @default.
• W2293449318 cites W2185806598 @default.

• next