FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2293828205> ?p ?o ?g. }

• W2293828205 endingPage "386" @default.
• W2293828205 startingPage "383" @default.
• W2293828205 abstract "FRET (Förster resonance energy transfer) involves the transfer of energy from an excited donor fluorophore to an acceptor molecule in a manner that is dependent on the distance between the two. A biochemistry laboratory experiment is described that teaches students how to use FRET to evaluate distance changes in biological molecules. Students measured the apparent FRET between donor and acceptor fluorophores located on the ends of several DNAs of unknown lengths, enabling them to order the DNAs according to size. In addition, students investigated site-specific DNA cleavage by restriction endonucleases, using loss of apparent FRET to determine which enzyme cut sites were present in each of the DNAs. After completing this experiment, students understood the inverse relationship between changes in FRET and changes in distance, and understood how changes in FRET could be used to monitor a conformational change in a molecule. As an extension to the experiment, a tutorial is included that uses the same DNAs to illustrate the ability of single-molecule FRET measurements to resolve heterogeneity in a sample, which cannot be done via more traditional ensemble measurements." @default.
• W2293828205 created "2016-06-24" @default.
• W2293828205 creator A5016305489 @default.
• W2293828205 creator A5019158392 @default.
• W2293828205 creator A5028445390 @default.
• W2293828205 creator A5053913775 @default.
• W2293828205 date "2015-12-21" @default.
• W2293828205 modified "2023-09-26" @default.
• W2293828205 title "Evaluating the Relationship between FRET Changes and Distance Changes Using DNA Length and Restriction Enzyme Specificity" @default.
• W2293828205 cites W1551325117 @default.
• W2293828205 cites W1911988712 @default.
• W2293828205 cites W1964142498 @default.
• W2293828205 cites W1983503767 @default.
• W2293828205 cites W2007327264 @default.
• W2293828205 cites W2013412105 @default.
• W2293828205 cites W2017617588 @default.
• W2293828205 cites W2034296563 @default.
• W2293828205 cites W2034999042 @default.
• W2293828205 cites W2039399143 @default.
• W2293828205 cites W2045642561 @default.
• W2293828205 cites W2063662362 @default.
• W2293828205 cites W2128671739 @default.
• W2293828205 cites W2132056215 @default.
• W2293828205 doi "https://doi.org/10.1021/acs.jchemed.5b00440" @default.
• W2293828205 hasPublicationYear "2015" @default.
• W2293828205 type Work @default.
• W2293828205 sameAs 2293828205 @default.
• W2293828205 citedByCount "8" @default.
• W2293828205 countsByYear W22938282052017 @default.
• W2293828205 countsByYear W22938282052018 @default.
• W2293828205 countsByYear W22938282052020 @default.
• W2293828205 countsByYear W22938282052021 @default.
• W2293828205 countsByYear W22938282052022 @default.
• W2293828205 crossrefType "journal-article" @default.
• W2293828205 hasAuthorship W2293828205A5016305489 @default.
• W2293828205 hasAuthorship W2293828205A5019158392 @default.
• W2293828205 hasAuthorship W2293828205A5028445390 @default.
• W2293828205 hasAuthorship W2293828205A5053913775 @default.
• W2293828205 hasConcept C121332964 @default.
• W2293828205 hasConcept C12554922 @default.
• W2293828205 hasConcept C128319531 @default.
• W2293828205 hasConcept C151730666 @default.
• W2293828205 hasConcept C159467904 @default.
• W2293828205 hasConcept C175156509 @default.
• W2293828205 hasConcept C178790620 @default.
• W2293828205 hasConcept C181500209 @default.
• W2293828205 hasConcept C184779094 @default.
• W2293828205 hasConcept C185592680 @default.
• W2293828205 hasConcept C26873012 @default.
• W2293828205 hasConcept C2778617687 @default.
• W2293828205 hasConcept C2779892579 @default.
• W2293828205 hasConcept C2781455991 @default.
• W2293828205 hasConcept C32909587 @default.
• W2293828205 hasConcept C43369102 @default.
• W2293828205 hasConcept C552990157 @default.
• W2293828205 hasConcept C55493867 @default.
• W2293828205 hasConcept C62520636 @default.
• W2293828205 hasConcept C86803240 @default.
• W2293828205 hasConcept C91881484 @default.
• W2293828205 hasConcept C96305047 @default.
• W2293828205 hasConceptScore W2293828205C121332964 @default.
• W2293828205 hasConceptScore W2293828205C12554922 @default.
• W2293828205 hasConceptScore W2293828205C128319531 @default.
• W2293828205 hasConceptScore W2293828205C151730666 @default.
• W2293828205 hasConceptScore W2293828205C159467904 @default.
• W2293828205 hasConceptScore W2293828205C175156509 @default.
• W2293828205 hasConceptScore W2293828205C178790620 @default.
• W2293828205 hasConceptScore W2293828205C181500209 @default.
• W2293828205 hasConceptScore W2293828205C184779094 @default.
• W2293828205 hasConceptScore W2293828205C185592680 @default.
• W2293828205 hasConceptScore W2293828205C26873012 @default.
• W2293828205 hasConceptScore W2293828205C2778617687 @default.
• W2293828205 hasConceptScore W2293828205C2779892579 @default.
• W2293828205 hasConceptScore W2293828205C2781455991 @default.
• W2293828205 hasConceptScore W2293828205C32909587 @default.
• W2293828205 hasConceptScore W2293828205C43369102 @default.
• W2293828205 hasConceptScore W2293828205C552990157 @default.
• W2293828205 hasConceptScore W2293828205C55493867 @default.
• W2293828205 hasConceptScore W2293828205C62520636 @default.
• W2293828205 hasConceptScore W2293828205C86803240 @default.
• W2293828205 hasConceptScore W2293828205C91881484 @default.
• W2293828205 hasConceptScore W2293828205C96305047 @default.
• W2293828205 hasFunder F4320337397 @default.
• W2293828205 hasIssue "2" @default.
• W2293828205 hasLocation W22938282051 @default.
• W2293828205 hasOpenAccess W2293828205 @default.
• W2293828205 hasPrimaryLocation W22938282051 @default.
• W2293828205 hasRelatedWork W191870946 @default.
• W2293828205 hasRelatedWork W2053633782 @default.
• W2293828205 hasRelatedWork W2054530395 @default.
• W2293828205 hasRelatedWork W2073064246 @default.
• W2293828205 hasRelatedWork W2088072996 @default.
• W2293828205 hasRelatedWork W2537649798 @default.
• W2293828205 hasRelatedWork W2539838344 @default.
• W2293828205 hasRelatedWork W2766666282 @default.
• W2293828205 hasRelatedWork W29063239 @default.
• W2293828205 hasRelatedWork W3215073180 @default.
• W2293828205 hasVolume "93" @default.

• next