Matches in SemOpenAlex for { <https://semopenalex.org/work/W2294014384> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2294014384 abstract "Mass spectrometry Imaging (MSI) was first reported for the direct analysis of intact proteins from tissue. The need for the identification of proteins and therefore their enzymatic digestion has become an essential step in the discovery of biomarkers from tissue. Structural identification is traditionally carried out after processing untargeted MS image data followed by further manual acquisitions either on the same or consecutive sections.A data independent MALDI imaging acquisition method is presented, where MS and MS/MS information are acquired within a single experiment, without any precursor selection or selection requirements. The precursors and the fragments are correlated post acquisition on the basis of their common drift time, which is further refined utilizing spatial distribution commonality.On-tissue digestion step was carried out where a trypsin solution was sprayed on tissue sections using a SunCollect nebulising spray device. After overnight incubation at 37°C, CHCA matrix was evenly applied with the same nebulising spray device. Data were acquired using a MALDI SYNAPT G2 instrument with tri-wave ion guide optics to separate ions according to their gas phase mobility.The MALDI imaging experiment was designed such that adjacent pixels had low and elevated collision energy applied in the Transfer T-Wave collision cell situated after the drift cell. The low energy function, comprising tryptic peptide precursor ion images, and the elevated energy function, providing fragmentation profiles ion images, were independently visualised within the Analysis tab of HDI software. Using the drift time alignment functionality, tryptic peptide precursors were associated with product ions that share similar drift times. For additional and supplementary refinement, another level of correlation was achieved on the basis of spatial distribution commonality. A .pkl file is generated for MASCOT database search where improvement of the MASCOT score was demonstrated." @default.
- W2294014384 created "2016-06-24" @default.
- W2294014384 creator A5023432101 @default.
- W2294014384 creator A5032246279 @default.
- W2294014384 creator A5048429407 @default.
- W2294014384 creator A5053162179 @default.
- W2294014384 creator A5061800453 @default.
- W2294014384 date "2013-05-01" @default.
- W2294014384 modified "2023-09-27" @default.
- W2294014384 title "Visualisation and Identification of Proteins Directly from a Single On-Tissue Tryptically Digested Sections Using MALDI Imaging HDMSE." @default.
- W2294014384 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3635284" @default.
- W2294014384 hasPublicationYear "2013" @default.
- W2294014384 type Work @default.
- W2294014384 sameAs 2294014384 @default.
- W2294014384 citedByCount "0" @default.
- W2294014384 crossrefType "journal-article" @default.
- W2294014384 hasAuthorship W2294014384A5023432101 @default.
- W2294014384 hasAuthorship W2294014384A5032246279 @default.
- W2294014384 hasAuthorship W2294014384A5048429407 @default.
- W2294014384 hasAuthorship W2294014384A5053162179 @default.
- W2294014384 hasAuthorship W2294014384A5061800453 @default.
- W2294014384 hasConcept C101092202 @default.
- W2294014384 hasConcept C111919701 @default.
- W2294014384 hasConcept C150394285 @default.
- W2294014384 hasConcept C15213710 @default.
- W2294014384 hasConcept C162356407 @default.
- W2294014384 hasConcept C162711632 @default.
- W2294014384 hasConcept C178790620 @default.
- W2294014384 hasConcept C181199279 @default.
- W2294014384 hasConcept C185592680 @default.
- W2294014384 hasConcept C191015642 @default.
- W2294014384 hasConcept C2780340462 @default.
- W2294014384 hasConcept C41008148 @default.
- W2294014384 hasConcept C43617362 @default.
- W2294014384 hasConcept C55493867 @default.
- W2294014384 hasConcept C70721500 @default.
- W2294014384 hasConcept C75280812 @default.
- W2294014384 hasConcept C86803240 @default.
- W2294014384 hasConceptScore W2294014384C101092202 @default.
- W2294014384 hasConceptScore W2294014384C111919701 @default.
- W2294014384 hasConceptScore W2294014384C150394285 @default.
- W2294014384 hasConceptScore W2294014384C15213710 @default.
- W2294014384 hasConceptScore W2294014384C162356407 @default.
- W2294014384 hasConceptScore W2294014384C162711632 @default.
- W2294014384 hasConceptScore W2294014384C178790620 @default.
- W2294014384 hasConceptScore W2294014384C181199279 @default.
- W2294014384 hasConceptScore W2294014384C185592680 @default.
- W2294014384 hasConceptScore W2294014384C191015642 @default.
- W2294014384 hasConceptScore W2294014384C2780340462 @default.
- W2294014384 hasConceptScore W2294014384C41008148 @default.
- W2294014384 hasConceptScore W2294014384C43617362 @default.
- W2294014384 hasConceptScore W2294014384C55493867 @default.
- W2294014384 hasConceptScore W2294014384C70721500 @default.
- W2294014384 hasConceptScore W2294014384C75280812 @default.
- W2294014384 hasConceptScore W2294014384C86803240 @default.
- W2294014384 hasLocation W22940143841 @default.
- W2294014384 hasOpenAccess W2294014384 @default.
- W2294014384 hasPrimaryLocation W22940143841 @default.
- W2294014384 hasRelatedWork W1410038390 @default.
- W2294014384 hasRelatedWork W1530269796 @default.
- W2294014384 hasRelatedWork W1969860457 @default.
- W2294014384 hasRelatedWork W1972020715 @default.
- W2294014384 hasRelatedWork W1983860480 @default.
- W2294014384 hasRelatedWork W2009505243 @default.
- W2294014384 hasRelatedWork W2016382203 @default.
- W2294014384 hasRelatedWork W2027475100 @default.
- W2294014384 hasRelatedWork W2062049401 @default.
- W2294014384 hasRelatedWork W2116134054 @default.
- W2294014384 hasRelatedWork W2136528923 @default.
- W2294014384 hasRelatedWork W2160107783 @default.
- W2294014384 hasRelatedWork W2175021072 @default.
- W2294014384 hasRelatedWork W2477482887 @default.
- W2294014384 hasRelatedWork W2540265159 @default.
- W2294014384 hasRelatedWork W2886456793 @default.
- W2294014384 hasRelatedWork W2917630616 @default.
- W2294014384 hasRelatedWork W2979111465 @default.
- W2294014384 hasRelatedWork W62270706 @default.
- W2294014384 hasRelatedWork W2599525623 @default.
- W2294014384 hasVolume "24" @default.
- W2294014384 isParatext "false" @default.
- W2294014384 isRetracted "false" @default.
- W2294014384 magId "2294014384" @default.
- W2294014384 workType "article" @default.