FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2294487613> ?p ?o ?g. }

• W2294487613 endingPage "1588" @default.
• W2294487613 startingPage "1574" @default.
• W2294487613 abstract "DARPin libraries, based on a Designed Ankyrin Repeat Protein consensus framework, are a rich source of binding partners for a wide variety of proteins. Their modular structure, stability, ease of in vitro selection and high production yields make DARPins an ideal starting point for further engineering. The X-ray structures of around 30 different DARPin complexes demonstrate their ability to facilitate crystallization of their target proteins by restricting flexibility and preventing undesired interactions of the target molecule. However, their small size (18 kDa), very hydrophilic surface and repetitive structure can limit the DARPins' ability to provide essential crystal contacts and their usefulness as a search model for addressing the crystallographic phase problem in molecular replacement. To optimize DARPins for their application as crystallization chaperones, rigid domain–domain fusions of the DARPins to larger proteins, proven to yield high-resolution crystal structures, were generated. These fusions were designed in such a way that they affect only one of the terminal capping repeats of the DARPin and do not interfere with residues involved in target binding, allowing to exchange at will the binding specificities of the DARPin in the fusion construct. As a proof of principle, we designed rigid fusions of a stabilized version of Escherichia coli TEM-1 β-lactamase to the C-terminal capping repeat of various DARPins in six different relative domain orientations. Five crystal structures representing four different fusion constructs, alone or in complex with the cognate target, show the predicted relative domain orientations and prove the validity of the concept." @default.
• W2294487613 created "2016-06-24" @default.
• W2294487613 creator A5022754180 @default.
• W2294487613 creator A5025006805 @default.
• W2294487613 creator A5029337710 @default.
• W2294487613 creator A5029902815 @default.
• W2294487613 creator A5083658123 @default.
• W2294487613 date "2016-04-01" @default.
• W2294487613 modified "2023-10-04" @default.
• W2294487613 title "DARPin-Based Crystallization Chaperones Exploit Molecular Geometry as a Screening Dimension in Protein Crystallography" @default.
• W2294487613 cites W1969649052 @default.
• W2294487613 cites W1976033992 @default.
• W2294487613 cites W1977315164 @default.
• W2294487613 cites W1981227353 @default.
• W2294487613 cites W1992291799 @default.
• W2294487613 cites W2005174659 @default.
• W2294487613 cites W2008892949 @default.
• W2294487613 cites W2009276920 @default.
• W2294487613 cites W2025259372 @default.
• W2294487613 cites W2027510502 @default.
• W2294487613 cites W2035503835 @default.
• W2294487613 cites W2043681747 @default.
• W2294487613 cites W2053991371 @default.
• W2294487613 cites W2055145326 @default.
• W2294487613 cites W2072828079 @default.
• W2294487613 cites W2080231167 @default.
• W2294487613 cites W2081912151 @default.
• W2294487613 cites W2084938664 @default.
• W2294487613 cites W2092803887 @default.
• W2294487613 cites W2095403873 @default.
• W2294487613 cites W2104477830 @default.
• W2294487613 cites W2112066436 @default.
• W2294487613 cites W2124026197 @default.
• W2294487613 cites W2126220191 @default.
• W2294487613 cites W2145923012 @default.
• W2294487613 cites W2146030191 @default.
• W2294487613 cites W2154644487 @default.
• W2294487613 cites W2155510074 @default.
• W2294487613 cites W2156414657 @default.
• W2294487613 cites W2163341755 @default.
• W2294487613 cites W2165238581 @default.
• W2294487613 cites W2170667639 @default.
• W2294487613 cites W2180229411 @default.
• W2294487613 cites W2201713963 @default.
• W2294487613 cites W4248872320 @default.
• W2294487613 doi "https://doi.org/10.1016/j.jmb.2016.03.002" @default.
• W2294487613 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26975886" @default.
• W2294487613 hasPublicationYear "2016" @default.
• W2294487613 type Work @default.
• W2294487613 sameAs 2294487613 @default.
• W2294487613 citedByCount "29" @default.
• W2294487613 countsByYear W22944876132017 @default.
• W2294487613 countsByYear W22944876132018 @default.
• W2294487613 countsByYear W22944876132019 @default.
• W2294487613 countsByYear W22944876132020 @default.
• W2294487613 countsByYear W22944876132021 @default.
• W2294487613 countsByYear W22944876132022 @default.
• W2294487613 crossrefType "journal-article" @default.
• W2294487613 hasAuthorship W2294487613A5022754180 @default.
• W2294487613 hasAuthorship W2294487613A5025006805 @default.
• W2294487613 hasAuthorship W2294487613A5029337710 @default.
• W2294487613 hasAuthorship W2294487613A5029902815 @default.
• W2294487613 hasAuthorship W2294487613A5083658123 @default.
• W2294487613 hasConcept C104317684 @default.
• W2294487613 hasConcept C113241181 @default.
• W2294487613 hasConcept C123894998 @default.
• W2294487613 hasConcept C144292202 @default.
• W2294487613 hasConcept C147816474 @default.
• W2294487613 hasConcept C181199279 @default.
• W2294487613 hasConcept C185592680 @default.
• W2294487613 hasConcept C2777166331 @default.
• W2294487613 hasConcept C40767141 @default.
• W2294487613 hasConcept C47701112 @default.
• W2294487613 hasConcept C54355233 @default.
• W2294487613 hasConcept C55493867 @default.
• W2294487613 hasConcept C70721500 @default.
• W2294487613 hasConcept C8010536 @default.
• W2294487613 hasConcept C86803240 @default.
• W2294487613 hasConceptScore W2294487613C104317684 @default.
• W2294487613 hasConceptScore W2294487613C113241181 @default.
• W2294487613 hasConceptScore W2294487613C123894998 @default.
• W2294487613 hasConceptScore W2294487613C144292202 @default.
• W2294487613 hasConceptScore W2294487613C147816474 @default.
• W2294487613 hasConceptScore W2294487613C181199279 @default.
• W2294487613 hasConceptScore W2294487613C185592680 @default.
• W2294487613 hasConceptScore W2294487613C2777166331 @default.
• W2294487613 hasConceptScore W2294487613C40767141 @default.
• W2294487613 hasConceptScore W2294487613C47701112 @default.
• W2294487613 hasConceptScore W2294487613C54355233 @default.
• W2294487613 hasConceptScore W2294487613C55493867 @default.
• W2294487613 hasConceptScore W2294487613C70721500 @default.
• W2294487613 hasConceptScore W2294487613C8010536 @default.
• W2294487613 hasConceptScore W2294487613C86803240 @default.
• W2294487613 hasFunder F4320320924 @default.
• W2294487613 hasIssue "8" @default.
• W2294487613 hasLocation W22944876131 @default.
• W2294487613 hasLocation W22944876132 @default.
• W2294487613 hasOpenAccess W2294487613 @default.

• next