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- W2294941861 abstract "The broad-range phospholipase C (PLC) from Listeria monocytogenes has been expressed using an intein expression system and characterized. This zinc metalloenzyme, similar to the homologous enzyme from Bacillus cereus, targets a wide range of lipid substrates. With monomeric substrates, the length of the hydrophobic acyl chain has significant impact on enzyme efficiency by affecting substrate affinity (Km). Based on a homology model of the enzyme to the B. cereus protein, several active site residue mutations were generated. While this PLC shares many of the mechanistic characteristics of the B. cereus PLC, a major difference is that the L. monocytogenes enzyme displays an acidic pH optimum regardless of substrate status (monomer, micelle, or vesicle). This unusual behavior might be advantageous for its role in the pathogenicity of L. monocytogenes." @default.
- W2294941861 created "2016-06-24" @default.
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- W2294941861 date "2016-06-01" @default.
- W2294941861 modified "2023-09-25" @default.
- W2294941861 title "Recombinant broad-range phospholipase C from Listeria monocytogenes exhibits optimal activity at acidic pH" @default.
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- W2294941861 doi "https://doi.org/10.1016/j.bbapap.2016.03.008" @default.
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