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- W2295531856 abstract "In this paper,the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit gene rbcL was cloned and analyzed from Chlorella sorokiniana CS-01 chloroplast genome.Sequencing analysis indicated that this fragment was 1428 bp in size.The com-plete open reading frame of Chlorella sorokiniana CS-01 rbcL gene is 1425bp encoding a polypeptide of 475 amino acids.The BLAST result shows that Chlorella sorokiniana CS-01 has the highest homologies with Chlorella sp.IFRPD 1018 with 99.2% sequence similarity.The BLAST result of 18S rDNA shows that Chlorella sorokiniana CS-01 has the highest homologies with Chlorella sorokiniana with 99.7% sequence similarity.Two phylogenetic trees of rbcL and 18S rDNA were constructed with the published rbcL and 18S rDNA se-quences of the relative microalga species.However,in the phylogenetic tree of 18S rDNA,the main branches are small,even if the branches were identified,the bootstrap values of branches are relatively weak,while in the phylogenetic tree of rbcL gene,the main branches are clear with high bootstrap values.This suggests that 18S rDNA gene is more conservative and evolves much slower than rbcL genes.Thus,using rbcL sequences in molecular systematics seems to be more appropriate for the phylogenetic analysis of closely related microalga than 18S rDNA." @default.
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- W2295531856 date "2010-01-01" @default.
- W2295531856 modified "2023-09-23" @default.
- W2295531856 title "Cloning and sequence analysis of rbcL gene from Chlorella sorokiniana and comparison with 18S rDNA gene in taxonomy." @default.
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