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- W2295536192 abstract "1,3-propanediol is an important chemical used as building block for the synthesis of highly promising polyesters such as polytrimethylene terephthalate. A genetically modified Klebsiella pneumoniae LDH526 can use glycerol as sole carbon source and produce 1,3-propanediol with the titer above 90 g/L. A key factor affecting the production of 1,3-propanediol by the mutant K. pneumoniae is the accurate control of the feeding of glycerol. To generate a robust and reproducible fermentation process of 1,3-propanediol, we designed and optimized an automatically feeding strategy of glycerol based on fermentation kinetics. By coupling the substrate feeding rate with easily observed variables -pH and fermentation time, we have achieved self-starting glycerol feeding and dynamic control of the glycerol concentration during the fermentation process. This automated system allowed us to generate a reproducible, consistent and operator-independent process from lab-scale to production scale. The final concentration of 1,3-propanediol was above 95 g/L after 72 h." @default.
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- W2295536192 date "2015-10-01" @default.
- W2295536192 modified "2023-10-15" @default.
- W2295536192 title "[Automatically feeding strategy for 1,3-propanediol fermentation of Klebsiella pneumoniae LDH526]." @default.
- W2295536192 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26964341" @default.
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