Matches in SemOpenAlex for { <https://semopenalex.org/work/W2295966940> ?p ?o ?g. }
- W2295966940 abstract "The culture conditions needed to maximize the production of laccase from Pholiota highlandensis myce- lia were investigated. Among the tested media for laccase production, Coriolus versicolor medium (CVM; 2% dextrose, 0.4% peptone, 0.6% yeast extract, 0.046% KH 2PO4, 0.1% K2HPO4, 0.05% MgSO4· 7H2O) showed the highest activity for the enzyme. Then, to optimize culture conditions for laccase activity, the influences of various carbon, nitrogen, phosphoru s, and inorganic salt sources in CVM were investigated. The optimum culture medium was 2% fructose, 0.4% peptone with 0.6% yeast ex- tract, 0.05% NaH 2PO4, and 0.05% MgSO 4∙7H2 O as carbon, nitrogen, phosphorus, and inorganic salt sources, respectively. Several aromatic compounds in the medium enhanced laccase activity to varying degrees. Guaiacol induced maximum laccase production, yielding 114.1 U/ml laccase activity after cul- tivation for 11 days at 25°C. The optimum pH and temperature for laccase production were 8.0 and 35°C, respectively. Native polyacrylamide-gel electrophoresis (PAGE) followed by laccase-activity staining with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the substrate was per- formed to identify the presence of laccase under the optimum conditions studied. Zymogram analysis of the supernatant culture showed an enzymatic band with a molecular mass of about 90 kDa." @default.
- W2295966940 created "2016-06-24" @default.
- W2295966940 creator A5020111504 @default.
- W2295966940 creator A5049256063 @default.
- W2295966940 creator A5069145788 @default.
- W2295966940 date "2015-06-30" @default.
- W2295966940 modified "2023-09-28" @default.
- W2295966940 title "Optimizing Culture Conditions to Maximize the Production of Laccase from Pholiota highlandensis" @default.
- W2295966940 cites W126978049 @default.
- W2295966940 cites W1966982831 @default.
- W2295966940 cites W1967242103 @default.
- W2295966940 cites W1977338669 @default.
- W2295966940 cites W1979996266 @default.
- W2295966940 cites W1988199321 @default.
- W2295966940 cites W1996626303 @default.
- W2295966940 cites W2008532143 @default.
- W2295966940 cites W2012491754 @default.
- W2295966940 cites W2013063245 @default.
- W2295966940 cites W2019230708 @default.
- W2295966940 cites W2030072141 @default.
- W2295966940 cites W2039047067 @default.
- W2295966940 cites W2049421223 @default.
- W2295966940 cites W2049674780 @default.
- W2295966940 cites W2059147057 @default.
- W2295966940 cites W2060542662 @default.
- W2295966940 cites W2066069148 @default.
- W2295966940 cites W2067622211 @default.
- W2295966940 cites W2077132244 @default.
- W2295966940 cites W2081214123 @default.
- W2295966940 cites W2090646579 @default.
- W2295966940 cites W2098740721 @default.
- W2295966940 cites W2102214200 @default.
- W2295966940 cites W2127668183 @default.
- W2295966940 cites W2131221831 @default.
- W2295966940 cites W2140133474 @default.
- W2295966940 cites W2141591389 @default.
- W2295966940 cites W2142587131 @default.
- W2295966940 cites W2159726158 @default.
- W2295966940 cites W2161739115 @default.
- W2295966940 cites W2162793734 @default.
- W2295966940 cites W2165966414 @default.
- W2295966940 cites W2351726600 @default.
- W2295966940 cites W2401545182 @default.
- W2295966940 cites W2406857371 @default.
- W2295966940 cites W2186049730 @default.
- W2295966940 doi "https://doi.org/10.5352/jls.2015.25.6.673" @default.
- W2295966940 hasPublicationYear "2015" @default.
- W2295966940 type Work @default.
- W2295966940 sameAs 2295966940 @default.
- W2295966940 citedByCount "0" @default.
- W2295966940 crossrefType "journal-article" @default.
- W2295966940 hasAuthorship W2295966940A5020111504 @default.
- W2295966940 hasAuthorship W2295966940A5049256063 @default.
- W2295966940 hasAuthorship W2295966940A5069145788 @default.
- W2295966940 hasBestOaLocation W22959669401 @default.
- W2295966940 hasConcept C100544194 @default.
- W2295966940 hasConcept C145890019 @default.
- W2295966940 hasConcept C181199279 @default.
- W2295966940 hasConcept C185592680 @default.
- W2295966940 hasConcept C2778004101 @default.
- W2295966940 hasConcept C2780199054 @default.
- W2295966940 hasConcept C2781031786 @default.
- W2295966940 hasConcept C2781401929 @default.
- W2295966940 hasConcept C31903555 @default.
- W2295966940 hasConcept C43617362 @default.
- W2295966940 hasConcept C55493867 @default.
- W2295966940 hasConcept C76935637 @default.
- W2295966940 hasConceptScore W2295966940C100544194 @default.
- W2295966940 hasConceptScore W2295966940C145890019 @default.
- W2295966940 hasConceptScore W2295966940C181199279 @default.
- W2295966940 hasConceptScore W2295966940C185592680 @default.
- W2295966940 hasConceptScore W2295966940C2778004101 @default.
- W2295966940 hasConceptScore W2295966940C2780199054 @default.
- W2295966940 hasConceptScore W2295966940C2781031786 @default.
- W2295966940 hasConceptScore W2295966940C2781401929 @default.
- W2295966940 hasConceptScore W2295966940C31903555 @default.
- W2295966940 hasConceptScore W2295966940C43617362 @default.
- W2295966940 hasConceptScore W2295966940C55493867 @default.
- W2295966940 hasConceptScore W2295966940C76935637 @default.
- W2295966940 hasLocation W22959669401 @default.
- W2295966940 hasOpenAccess W2295966940 @default.
- W2295966940 hasPrimaryLocation W22959669401 @default.
- W2295966940 hasRelatedWork W2004741284 @default.
- W2295966940 hasRelatedWork W2032006250 @default.
- W2295966940 hasRelatedWork W2049041345 @default.
- W2295966940 hasRelatedWork W2061741055 @default.
- W2295966940 hasRelatedWork W2075561464 @default.
- W2295966940 hasRelatedWork W2088682822 @default.
- W2295966940 hasRelatedWork W2089203172 @default.
- W2295966940 hasRelatedWork W2119731735 @default.
- W2295966940 hasRelatedWork W2124238908 @default.
- W2295966940 hasRelatedWork W2442960958 @default.
- W2295966940 hasRelatedWork W2472131530 @default.
- W2295966940 hasRelatedWork W2518520166 @default.
- W2295966940 hasRelatedWork W2738225524 @default.
- W2295966940 hasRelatedWork W2905538080 @default.
- W2295966940 hasRelatedWork W2952894494 @default.
- W2295966940 hasRelatedWork W3081611477 @default.
- W2295966940 hasRelatedWork W3143704477 @default.
- W2295966940 hasRelatedWork W3171158028 @default.