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- W2296018167 abstract "Human skin epithelium (NCTC 3075), monkey kidney epithelium (NCTC 3526-LLCMK2), and Earle's strain L C3H mouse fibrosarcoma cells (NCTC 2071), cultured in chemically defined medium NCTC 109 with no added proteins for 2 to 6 years, were frozen to −196° C in this medium after incorporation of 6 or 12 percent glycerol. The cells were subsequently thawed and cultured in this medium with no apparent alterations. A slow rate of freezing, 1° to 2° C per minute in a dry-ice ethanol bath, was used until −25°C was reached. Then the ampoules of frozen cell suspension were plunged into liquid nitrogen for storage. The cells were thawed within 1 minute or less by immersion and agitation of the ampoules in water at 40° C. The human skin and monkey kidney strains were capable of proliferation, provided the concentration of glycerol was reduced to no more than 1 percent in the cell suspension by dilution with medium NCTC 109 after the thawing. NCTC 2071 cells could be recovered after freezing with 12 percent glycerol only when the glycerol was removed in gradients of 1 percent with 3 to 4 minutes allowed between each gradient until less than 1 percent remained in the cell suspension. They could, however, proliferate when recovered from medium NCTC 109 and 6 percent glycerol, irrespective of the rate of glycerol removal. These data show that NCTC 2071 (strain L) cells are more sensitive to the manipulations required in freezing procedures than the other two lines of cells. With appropriate modifications in existing freezing procedures, the recovery of cells in chemically defined medium is possible. Further studies on the long-term preservation of these and other cell lines in liquid nitrogen are in progress." @default.
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- W2296018167 date "1962-10-01" @default.
- W2296018167 modified "2023-09-27" @default.
- W2296018167 title "Recovery From Liquid-Nitrogen Temperature of Established Cell Lines Frozen in Chemically Defined Medium" @default.
- W2296018167 doi "https://doi.org/10.1093/jnci/29.4.749" @default.
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