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- W2296164418 abstract "Recycling of the vacuolar proton (H+) pumping ATPase (V-ATPase) between intracellular vesicles and the plasma membrane in H+-secreting cells is central to acid-base regulation by the kidney. Collecting duct (CD) A-type intercalated cells (A-IC) respond to systemic acidosis by accumulating the V-ATPase in their apical membrane, which correlates with higher rates of H+ secretion and increased microvillar extension. We show that CPT-cAMP infused in vivo results in an almost two-fold increase in apical V-ATPase accumulation in A-IC within 15 min, and that these cells develop an extensive array of apical microvilli. The cAMP effect on A-IC is direct, as shown in vitro in cells isolated from the medulla of transgenic mice expressing EGFP in IC (driven by the V-ATPase B1-subunit promoter). 3D-reconstructions of confocal images showed that cAMP induced a dramatic, time dependent growth of microvilli in isolated IC. These morphological changes were paralleled by increased cAMP-mediated H+ extrusion rates by A-IC in isolated outer medullary CD measured using the ratiometric probe BCECF. These results support the idea that cAMP, generated by the soluble adenylyl cyclase (sAC - which is enriched in IC) or other effectors, is part of the signal transduction mechanism for acid-base sensing and V-ATPase trafficking in IC. Funding source: NIH DK-73266, DK-42956, DK-38452 and HD-40793." @default.
- W2296164418 created "2016-06-24" @default.
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- W2296164418 date "2010-04-01" @default.
- W2296164418 modified "2023-10-18" @default.
- W2296164418 title "V‐ATPase membrane accumulation and activity are stimulated by cAMP in A‐type intercalated cells" @default.
- W2296164418 doi "https://doi.org/10.1096/fasebj.24.1_supplement.1024.6" @default.
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