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- W2296648052 abstract "In an effort to regulate the mammalian cell behavior in entrapment with a gel, we have functionalized hydrogels with the putative cell-binding (-Arg-Gly-Asp-) (RGD) domain. An adhesion molecule of Gly-Arg-Gly-Asp-Ser (GRGDS) peptides, a cell recognition ligand, was induced into thermo-reversible hydrogels, composed of N-isopropylacrylamide with small amounts of acrylic acid (typically 2-5 mol% in feed), as a biomimetic extracellular matrix (ECM). The GRGDS containing a p(NiPAAm-co-AAc) copolymer gel was studied in vitro for its ability to promote the spreading and viability of cells by introducing a GRGDS sequence. Hydrogel with no adhesion molecule was a poor ECM for adhesion, permiting spreading of only 3% of the seeded cells for 36 h. By immobilizing the peptide linkage into the hydrogel, the conjugation of RGD promoted 50% of proliferation for 36 h. However, the GREDS sequence, nonadhesive peptide linkage, conjugated hydrogel showed only 5% of the seeded cell for the same time period. In addition, with the serum-free medium, only GRGDS peptides conjugated to hydrogel was able to promote cell spreading, while there was no cell proliferation in the hydrogel without GRGDS. Thus, the GRGDS peptide-conjugated thermo-reversible hydrogel specifically mediated the cell spreading. This result suggests that utilization of peptide sequences conjugating with the cell-adhesive motifs can enhance the degree of cell surface interaction and influence the long-term formation of ECM in vitro." @default.
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- W2296648052 date "2001-01-01" @default.
- W2296648052 modified "2023-09-28" @default.
- W2296648052 title "Articles : Certification of Fibroblast Cell Adhesion and Spreading Mediated by Arg - Gly - Asp ( RGD ) Sequence on Thermo - Reversible Hydrogel" @default.
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